6ITZ

Peroxiredoxin from Thermococcus kodakaraensis

6ITZ の概要

• エントリーDOI: 10.2210/pdb6itz/pdb
• 分子名称: Peroxiredoxin (1 entity in total)
• 機能のキーワード: hydrogen peroxide, oxidoreductase, dodecamer
• 由来する生物種: Thermococcus kodakarensis KOD1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49354.73

構造登録者

Nakamura, T.,Himiyama, T. (登録日: 2018-11-27, 公開日: 2019-03-06, 最終更新日: 2024-10-23)

主引用文献

Himiyama, T.,Oshima, M.,Uegaki, K.,Nakamura, T.
Distinct molecular assembly of homologous peroxiredoxins from Pyrococcus horikoshii and Thermococcus kodakaraensis.
J.Biochem., 166:89-95, 2019

PubMed Abstract: Peroxiredoxins from Pyrococcus horikoshii (PhPrx) and Thermococcus kodakaraensis (TkPrx) are highly homologous proteins sharing 196 of the 216 residues. We previously reported a pentagonal ring-type decameric structure of PhPrx. Here, we present the crystal structure of TkPrx. Despite their homology, unlike PhPrx, the quaternary structure of TkPrx was found to be a dodecamer comprised of six homodimers arranged in a hexagonal ring-type assembly. The possibility of the redox-dependent conversion of the molecular assembly, which had been observed in PhPrx, was excluded for TkPrx based on the crystal structure of a mutant in which all of the cysteine residues were substituted with serine. The monomer structures of the dodecameric TkPrx and decameric PhPrx coincided well, but there was a slight difference in the relative orientation of the two domains. Molecular assembly of PhPrx and TkPrx in solution evaluated by gel-filtration chromatography was consistent with the crystallographic results. For both PhPrx and TkPrx, the gel-filtration elution volume slightly increased with a decrease in the protein concentration, suggesting the existence of an equilibrium state between the decameric/dodecameric ring and lower-order assembly. This structural assembly difference between highly homologous Prxs suggests a significant influence of quaternary structure on function, worthy of further exploration.

PubMed: 30796432
DOI: 10.1093/jb/mvz013

実験手法

X-RAY DIFFRACTION (2.96 Å)