6ITW

Crystal structure of Atu4351 from Agrobacterium tumefaciens

6ITW の概要

• エントリーDOI: 10.2210/pdb6itw/pdb
• 分子名称: Type VI immunity protein Atu4351 (2 entities in total)
• 機能のキーワード: type six secretion system;effector-immunity pair; tdi1; gad-like domain; duf1851 domain, antitoxin
• 由来する生物種: Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26357.35

構造登録者

Shi, L.,Gao, Z.,Zhang, H.,Dong, Y. (登録日: 2018-11-26, 公開日: 2019-03-20, 最終更新日: 2024-10-16)

主引用文献

Shi, L.,Gao, Z.,Zhang, T.,Zhang, H.,Dong, Y.
Crystal structure of the type VI immunity protein Tdi1 (Atu4351) from Agrobacterium tumefaciens.
Acta Crystallogr F Struct Biol Commun, 75:153-158, 2019

PubMed Abstract: The type VI secretion system (T6SS) is a novel multiprotein needle-like apparatus that is distributed widely in Gram-negative bacteria. Bacteria harboring T6SSs inject various effectors into both eukaryotic and prokaryotic cells for interspecies competition or virulence-related processes. The toxicities of the effectors can be neutralized by their cognate immunity proteins. Tde1 (Atu4350)-Tdi1 (Atu4351) has recently been characterized as a T6SS effector-immunity pair in the soil bacterium Agrobacterium tumefaciens and the neutralization mechanism remains unknown. Here, the crystal structure of the immunity protein Tdi1 was determined at 2.40 Å resolution by the single-wavelength anomalous dispersion method. Structural analysis suggested that it is composed of a GAD-like domain and an inserted DUF1851 domain, and both domains show low structural similarities to known structures. There is a positive groove mainly located in the GAD-like domain that may be associated with nucleotide binding. The structure provides a basis for further study of the positive groove as a potential active site.

PubMed: 30839288
DOI: 10.1107/S2053230X19000815

実験手法

X-RAY DIFFRACTION (2.4 Å)