6ITH

Structure of the transmembrane domain of syndecan 2 in micelles

6ITH の概要

• エントリーDOI: 10.2210/pdb6ith/pdb
• 分子名称: Syndecan-2 (1 entity in total)
• 機能のキーワード: membrane protein, dpc, micelles
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4715.66

構造登録者

Li, Q.,Ng, H.Q.,Kang, C. (登録日: 2018-11-23, 公開日: 2019-02-27, 最終更新日: 2024-05-15)

主引用文献

Li, Q.,Ng, H.Q.,Kang, C.
Secondary structure and topology of the transmembrane domain of Syndecan-2 in detergent micelles.
FEBS Lett., 593:554-561, 2019

PubMed Abstract: Syndecans are single-span membrane proteins playing important roles in cell-cell and cell-matrix interactions. The transmembrane domain of syndecans is critical for signal transduction across the cell membrane. Here, the structure of the transmembrane domain of syndecan-2 in detergent micelles was investigated using solution NMR spectroscopy. Backbone resonance assignment was obtained, and NMR studies show that the transmembrane domain forms a helix in detergent micelles, which is also supported by the hydrogen and deuterium exchange experiment. A study of the dynamics revealed the rigid structure of the transmembrane domain formed in solution, and paramagnetic relaxation enhancement defined the topology of the transmembrane domain in detergent micelles. This structural analysis may facilitate a better understanding of the role of the syndecan-2 transmembrane domain in signal transduction.

PubMed: 30742705
DOI: 10.1002/1873-3468.13335

実験手法

SOLUTION NMR