6ITF

Icosahedral asymmetric unit (iASU) model of the less refined, coarse part of FHV eluted particle

6ITF の概要

• エントリーDOI: 10.2210/pdb6itf/pdb
• EMDBエントリー: 9730 9732
• 分子名称: CAPSID PROTEIN BETA (1 entity in total)
• 機能のキーワード: flock house virus, disassembly intermediate, asymmetric reconstruction, virus
• 由来する生物種: Flock house virus (FHV)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 118102.07

構造登録者

Banerjee, M.,Azad, K. (登録日: 2018-11-22, 公開日: 2019-08-28, 最終更新日: 2024-03-27)

主引用文献

Azad, K.,Banerjee, M.
Structural Dynamics of Nonenveloped Virus Disassembly Intermediates.
J.Virol., 93:-, 2019

PubMed Abstract: The stability of icosahedral viruses is crucial for protecting the viral genome during transit; however, successful infection requires eventual disassembly of the capsid. A comprehensive understanding of how stable, uniform icosahedrons disassemble remains elusive, mainly due to the complexities involved in isolating transient intermediates. We utilized incremental heating to systematically characterize the disassembly pathway of a model nonenveloped virus and identified an intriguing link between virus maturation and disassembly. Further, we isolated and characterized two intermediates by cryo-electron microscopy and three-dimensional reconstruction, without imposing icosahedral symmetry. The first intermediate displayed a series of major, asymmetric alterations, whereas the second showed that the act of genome release, through the 2-fold axis, is actually confined to a small section on the capsid. Our study thus presents a comprehensive structural analysis of nonenveloped virus disassembly and emphasizes the asymmetric nature of programmed conformational changes. Disassembly or uncoating of an icosahedral capsid is a crucial step during infection by nonenveloped viruses. However, the dynamic and transient nature of the disassembly process makes it challenging to isolate intermediates in a temporal, stepwise manner for structural characterization. Using controlled, incremental heating, we isolated two disassembly intermediates: "eluted particles" and "puffed particles" of an insect nodavirus, Flock House virus (FHV). Cryo-electron microscopy and three-dimensional reconstruction of the FHV disassembly intermediates indicated that disassembly-related conformational alterations are minimally global and largely local, leading to asymmetry in the particle and eventual genome release without complete disintegration of the icosahedron.

PubMed: 31484752
DOI: 10.1128/JVI.01115-19

実験手法

ELECTRON MICROSCOPY (4.7 Å)