6IQG

X-ray crystal structure of Fc and peptide complex

6IQG の概要

• エントリーDOI: 10.2210/pdb6iqg/pdb
• 分子名称: Immunoglobulin gamma-1 heavy chain, 18-mer peptide G(HCS)DCAYHRGELVWCT(HCS)H(NH2), 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: fc, complex, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 54504.43

構造登録者

Adachi, M.,Ito, Y. (登録日: 2018-11-08, 公開日: 2019-02-13, 最終更新日: 2024-11-13)

主引用文献

Kishimoto, S.,Nakashimada, Y.,Yokota, R.,Hatanaka, T.,Adachi, M.,Ito, Y.
Site-Specific Chemical Conjugation of Antibodies by Using Affinity Peptide for the Development of Therapeutic Antibody Format.
Bioconjug. Chem., 30:698-702, 2019

PubMed Abstract: Artificially modified IgG molecules are increasingly utilized in industrial and clinical applications. In the present study, the method of chemical conjugation by affinity peptide (CCAP) for site-specific chemical modification has been developed by using a peptide that bound with high affinity to human IgG-Fc. This method enabled a rapid modification of a specific residue (Lys248 on Fc) in a one-step reaction under mild condition to form a stable amide bond between the peptide and Fc. The monovalent peptide-IgG conjugate not only maintained complete antigen binding but also bound to Fc receptors (FcRn, FcγRI, and FcγRIIIa), indicating that it is a suitable conjugate form that can be further developed into highly functional antibody therapeutics. CCAP was applied for the preparation of an antibody-drug conjugate and a bispecific antibody to demonstrate the usefulness of this method.

PubMed: 30606013
DOI: 10.1021/acs.bioconjchem.8b00865

実験手法

X-RAY DIFFRACTION (2.998 Å)