6IK4

A Novel M23 Metalloprotease Pseudoalterin from Deep-sea

6IK4 の概要

• エントリーDOI: 10.2210/pdb6ik4/pdb
• 分子名称: Elastinolytic metalloprotease, ZINC ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: m23 family, elastase, peptidoglycan, hydrolase
• 由来する生物種: Pseudoalteromonas sp. CF6-2
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19547.44

構造登録者

Zhao, H.L.,Tang, B.L.,Yang, J.,Chen, X.L.,Zhang, Y.Z. (登録日: 2018-10-14, 公開日: 2019-10-16, 最終更新日: 2024-10-09)

主引用文献

Tang, B.L.,Yang, J.,Chen, X.L.,Wang, P.,Zhao, H.L.,Su, H.N.,Li, C.Y.,Yu, Y.,Zhong, S.,Wang, L.,Lidbury, I.,Ding, H.,Wang, M.,McMinn, A.,Zhang, X.Y.,Chen, Y.,Zhang, Y.Z.
A predator-prey interaction between a marine Pseudoalteromonas sp. and Gram-positive bacteria.
Nat Commun, 11:285-285, 2020

PubMed Abstract: Predator-prey interactions play important roles in the cycling of marine organic matter. Here we show that a Gram-negative bacterium isolated from marine sediments (Pseudoalteromonas sp. strain CF6-2) can kill Gram-positive bacteria of diverse peptidoglycan (PG) chemotypes by secreting the metalloprotease pseudoalterin. Secretion of the enzyme requires a Type II secretion system. Pseudoalterin binds to the glycan strands of Gram positive bacterial PG and degrades the PG peptide chains, leading to cell death. The released nutrients, including PG-derived D-amino acids, can then be utilized by strain CF6-2 for growth. Pseudoalterin synthesis is induced by PG degradation products such as glycine and glycine-rich oligopeptides. Genes encoding putative pseudoalterin-like proteins are found in many other marine bacteria. This study reveals a new microbial interaction in the ocean.

PubMed: 31941905
DOI: 10.1038/s41467-019-14133-x

実験手法

X-RAY DIFFRACTION (1.9 Å)