6IIJ

Cryo-EM structure of CV-A10 mature virion

Summary for 6IIJ

• Entry DOI: 10.2210/pdb6iij/pdb
• EMDB information: 9674
• Descriptor: VP1, VP2, VP3, ... (5 entities in total)
• Functional Keywords: coxsackievirus a10, mature virion, viral protein
• Biological source: Coxsackievirus A10; More
• Total number of polymer chains: 4
• Total formula weight: 94780.46

Authors

Chen, J.H.,Ye, X.H.,Cong, Y.,Huang, Z. (deposition date: 2018-10-06, release date: 2018-11-07, Last modification date: 2024-03-27)

Primary citation

Chen, J.,Ye, X.,Zhang, X.Y.,Zhu, Z.,Zhang, X.,Xu, Z.,Ding, Z.,Zou, G.,Liu, Q.,Kong, L.,Jiang, W.,Zhu, W.,Cong, Y.,Huang, Z.
Coxsackievirus A10 atomic structure facilitating the discovery of a broad-spectrum inhibitor against human enteroviruses.
Cell Discov, 5:4-4, 2019

PubMed Abstract: Coxsackievirus A10 (CV-A10) belongs to the species A and is a causative agent of hand, foot, and mouth disease. Here we present cryo-EM structures of CV-A10 mature virion and native empty particle (NEP) at 2.84 and 3.12 Å, respectively. Our CV-A10 mature virion structure reveals a density corresponding to a lipidic pocket factor of 18 carbon atoms in the hydrophobic pocket formed within viral protein 1. By structure-guided high-throughput drug screening and subsequent verification in cell-based infection-inhibition assays, we identified four compounds that inhibited CV-A10 infection in vitro. These compounds represent a new class of anti-enteroviral drug leads. Notably, one of the compounds, ICA135, also exerted broad-spectrum inhibitory effects on a number of representative viruses from all four species (A-D) of human enteroviruses. Our findings should facilitate the development of broadly effective drugs and vaccines for enterovirus infections.

PubMed: 30652025
DOI: 10.1038/s41421-018-0073-7

Experimental method

ELECTRON MICROSCOPY (2.84 Å)