6IGH

Crystal structure of FT condition3

6IGH の概要

• エントリーDOI: 10.2210/pdb6igh/pdb
• 分子名称: Protein FLOWERING LOCUS T, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: flowering control, plant protein
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19682.17

構造登録者

Watanabe, S.,Nakamura, Y.,Kanehara, K.,Inaba, K. (登録日: 2018-09-25, 公開日: 2019-12-25, 最終更新日: 2023-11-22)

主引用文献

Nakamura, Y.,Lin, Y.C.,Watanabe, S.,Liu, Y.C.,Katsuyama, K.,Kanehara, K.,Inaba, K.
High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering.
Iscience, 21:577-586, 2019

PubMed Abstract: Arabidopsis FLOWERING LOCUS T (FT) is a pivotal component of florigen, a long-range mobile flowering signal. Here, we determined the 1.0 Å-resolution crystal structure of FT, a significantly higher-resolution crystal structure of FT than previously reported one (2.6 Å). The present crystallographic studies revealed 4 alternative configurations with the precise location of the surrounding water molecules. Using this structural data, computational docking simulation predicted the putative binding sites for phosphatidylcholine (PC), an endogenous ligand that interacts with FT to modulate flowering time. In vitro reconstitution of the lipid-protein interaction showed that mutations at two of the predicted sites significantly compromised the lipid binding ability of FT. In planta, one of the mutant FT proteins significantly affected FT function in flowering, emphasizing the involvement of PC binding in modulating FT function. Our structural, biochemical, and transgenic analyses reveal the molecular mechanism of PC binding in FT-mediated flowering time control.

PubMed: 31726375
DOI: 10.1016/j.isci.2019.10.045

実験手法

X-RAY DIFFRACTION (1.01 Å)