6IEW

The crystal structure of the dNxf2 UBA domain in complex with Panoramix

6IEW の概要

• エントリーDOI: 10.2210/pdb6iew/pdb
• 分子名称: Fusion protein of Nuclear RNA export factor 2 and Protein panoramix, GLYCEROL (3 entities in total)
• 機能のキーワード: pirna, protein binding
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10340.64

構造登録者

Huang, Y.,Cheng, S. (登録日: 2018-09-17, 公開日: 2019-08-21, 最終更新日: 2024-03-27)

主引用文献

Zhao, K.,Cheng, S.,Miao, N.,Xu, P.,Lu, X.,Zhang, Y.,Wang, M.,Ouyang, X.,Yuan, X.,Liu, W.,Lu, X.,Zhou, P.,Gu, J.,Zhang, Y.,Qiu, D.,Jin, Z.,Su, C.,Peng, C.,Wang, J.H.,Dong, M.Q.,Wan, Y.,Ma, J.,Cheng, H.,Huang, Y.,Yu, Y.
A Pandas complex adapted for piRNA-guided transcriptional silencing and heterochromatin formation.
Nat.Cell Biol., 21:1261-1272, 2019

PubMed Abstract: The repression of transposons by the Piwi-interacting RNA (piRNA) pathway is essential to protect animal germ cells. In Drosophila, Panoramix enforces transcriptional silencing by binding to the target-engaged Piwi-piRNA complex, although the precise mechanisms by which this occurs remain elusive. Here, we show that Panoramix functions together with a germline-specific paralogue of a nuclear export factor, dNxf2, and its cofactor dNxt1 (p15), to suppress transposon expression. The transposon RNA-binding protein dNxf2 is required for animal fertility and Panoramix-mediated silencing. Transient tethering of dNxf2 to nascent transcripts leads to their nuclear retention. The NTF2 domain of dNxf2 competes dNxf1 (TAP) off nucleoporins, a process required for proper RNA export. Thus, dNxf2 functions in a Panoramix-dNxf2-dependent TAP/p15 silencing (Pandas) complex that counteracts the canonical RNA exporting machinery and restricts transposons to the nuclear peripheries. Our findings may have broader implications for understanding how RNA metabolism modulates heterochromatin formation.

PubMed: 31570835
DOI: 10.1038/s41556-019-0396-0

実験手法

X-RAY DIFFRACTION (1.5 Å)