6IDV

Peptide Asparaginyl Ligases from Viola yedoensis

6IDV の概要

• エントリーDOI: 10.2210/pdb6idv/pdb
• 分子名称: Peptide Asparaginyl Ligases, alpha-L-fucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: peptide ligase, pal, aep, ligase
• 由来する生物種: Viola philippica
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 108530.89

構造登録者

El Sahili, A.,Hu, S.,Lescar, J. (登録日: 2018-09-11, 公開日: 2019-05-15, 最終更新日: 2024-11-20)

主引用文献

Hemu, X.,El Sahili, A.,Hu, S.,Wong, K.,Chen, Y.,Wong, Y.H.,Zhang, X.,Serra, A.,Goh, B.C.,Darwis, D.A.,Chen, M.W.,Sze, S.K.,Liu, C.F.,Lescar, J.,Tam, J.P.
Structural determinants for peptide-bond formation by asparaginyl ligases.
Proc.Natl.Acad.Sci.USA, 116:11737-11746, 2019

PubMed Abstract: Asparaginyl endopeptidases (AEPs) are cysteine proteases which break Asx (Asn/Asp)-Xaa bonds in acidic conditions. Despite sharing a conserved overall structure with AEPs, certain plant enzymes such as butelase 1 act as a peptide asparaginyl ligase (PAL) and catalyze Asx-Xaa bond formation in near-neutral conditions. PALs also serve as macrocyclases in the biosynthesis of cyclic peptides. Here, we address the question of how a PAL can function as a ligase rather than a protease. Based on sequence homology of butelase 1, we identified AEPs and PALs from the cyclic peptide-producing plants () and () of the Violaceae family. Using a crystal structure of a PAL obtained at 2.4-Å resolution coupled to mutagenesis studies, we discovered ligase-activity determinants flanking the S1 site, namely LAD1 and LAD2 located around the S2 and S1' sites, respectively, which modulate ligase activity by controlling the accessibility of water or amine nucleophile to the -ester intermediate. Recombinantly expressed PAL1-3, predicted to be PALs, were confirmed to be ligases by functional studies. In addition, mutagenesis studies on PAL1-3, AEP1, and AEP supported our prediction that LAD1 and LAD2 are important for ligase activity. In particular, mutagenesis targeting LAD2 selectively enhanced the ligase activity of PAL3 and converted the protease AEP into a ligase. The definition of structural determinants required for ligation activity of the asparaginyl ligases presented here will facilitate genomic identification of PALs and engineering of AEPs into PALs.

PubMed: 31123145
DOI: 10.1073/pnas.1818568116

実験手法

X-RAY DIFFRACTION (2.4 Å)