6IDI
Cryo-EM structure of Immature Dengue virus serotype 3 in complex with human antibody 1H10 Fab at pH 8.0.
6IDI の概要
エントリーDOI | 10.2210/pdb6idi/pdb |
EMDBエントリー | 9649 9650 9651 |
分子名称 | Envelope protein, Premembrane protein, Fab 1H10 heavy chain (V-region), ... (4 entities in total) |
機能のキーワード | immature dengue virus, human antibody, virus |
由来する生物種 | Dengue virus 3 詳細 |
タンパク質・核酸の鎖数 | 12 |
化学式量合計 | 299461.51 |
構造登録者 | Wirawan, M.,Fibriansah, G.,Ng, T.S.,Zhang, Q.,Kostyuchenko, V.A.,Shi, J.,Lok, S.M. (登録日: 2018-09-10, 公開日: 2018-12-12, 最終更新日: 2024-03-27) |
主引用文献 | Wirawan, M.,Fibriansah, G.,Marzinek, J.K.,Lim, X.X.,Ng, T.S.,Sim, A.Y.L.,Zhang, Q.,Kostyuchenko, V.A.,Shi, J.,Smith, S.A.,Verma, C.S.,Anand, G.,Crowe Jr., J.E.,Bond, P.J.,Lok, S.M. Mechanism of Enhanced Immature Dengue Virus Attachment to Endosomal Membrane Induced by prM Antibody. Structure, 27:253-267.e8, 2019 Cited by PubMed Abstract: Dengue virus (DENV) particles are released from cells in different maturation states. Fully immature DENV (immDENV) is generally non-infectious, but can become infectious when complexed with anti-precursor membrane (prM) protein antibodies. It is unknown how anti-prM antibody-coated particles can undergo membrane fusion since the prM caps the envelope (E) protein fusion loop. Here, we determined cryoelectron microscopy (cryo-EM) maps of the immDENV:anti-prM complex at different pH values, mimicking the extracellular (pH 8.0) or endosomal (pH 5.0) environments. At pH 5.0, there are two structural classes with fewer antibodies bound than at pH 8.0. These classes may represent different maturation states. Molecular simulations, together with the measured high-affinity pr:antibody interaction (versus the weak pr:E interaction) and also the low pH cryo-EM structures, suggest how antibody:pr complex can dislodge from the E protein at low pH. This exposes the E protein fusion loop enhancing virus interaction with endosomes. PubMed: 30471923DOI: 10.1016/j.str.2018.10.009 主引用文献が同じPDBエントリー |
実験手法 | ELECTRON MICROSCOPY (12 Å) |
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