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6IDI

Cryo-EM structure of Immature Dengue virus serotype 3 in complex with human antibody 1H10 Fab at pH 8.0.

6IDI の概要
エントリーDOI10.2210/pdb6idi/pdb
EMDBエントリー9649 9650 9651
分子名称Envelope protein, Premembrane protein, Fab 1H10 heavy chain (V-region), ... (4 entities in total)
機能のキーワードimmature dengue virus, human antibody, virus
由来する生物種Dengue virus 3
詳細
タンパク質・核酸の鎖数12
化学式量合計299461.51
構造登録者
Wirawan, M.,Fibriansah, G.,Ng, T.S.,Zhang, Q.,Kostyuchenko, V.A.,Shi, J.,Lok, S.M. (登録日: 2018-09-10, 公開日: 2018-12-12, 最終更新日: 2024-03-27)
主引用文献Wirawan, M.,Fibriansah, G.,Marzinek, J.K.,Lim, X.X.,Ng, T.S.,Sim, A.Y.L.,Zhang, Q.,Kostyuchenko, V.A.,Shi, J.,Smith, S.A.,Verma, C.S.,Anand, G.,Crowe Jr., J.E.,Bond, P.J.,Lok, S.M.
Mechanism of Enhanced Immature Dengue Virus Attachment to Endosomal Membrane Induced by prM Antibody.
Structure, 27:253-267.e8, 2019
Cited by
PubMed Abstract: Dengue virus (DENV) particles are released from cells in different maturation states. Fully immature DENV (immDENV) is generally non-infectious, but can become infectious when complexed with anti-precursor membrane (prM) protein antibodies. It is unknown how anti-prM antibody-coated particles can undergo membrane fusion since the prM caps the envelope (E) protein fusion loop. Here, we determined cryoelectron microscopy (cryo-EM) maps of the immDENV:anti-prM complex at different pH values, mimicking the extracellular (pH 8.0) or endosomal (pH 5.0) environments. At pH 5.0, there are two structural classes with fewer antibodies bound than at pH 8.0. These classes may represent different maturation states. Molecular simulations, together with the measured high-affinity pr:antibody interaction (versus the weak pr:E interaction) and also the low pH cryo-EM structures, suggest how antibody:pr complex can dislodge from the E protein at low pH. This exposes the E protein fusion loop enhancing virus interaction with endosomes.
PubMed: 30471923
DOI: 10.1016/j.str.2018.10.009
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (12 Å)
構造検証レポート
Validation report summary of 6idi
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-01-29に公開中

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