6IBW

Crh5 transglycosylase in complex with NAG

「5NDL」から置き換えられました

6IBW の概要

• エントリーDOI: 10.2210/pdb6ibw/pdb
• 関連するPDBエントリー: 6IBU
• 分子名称: Probable glycosidase crf1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: cross-link, transglycosylase, gh16, cell wall assembly, hydrolase
• 由来する生物種: Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55704.65

構造登録者

Fang, W.,Bartual, S.G.,van Aalten, D.M.F. (登録日: 2018-12-01, 公開日: 2019-02-27, 最終更新日: 2024-11-13)

主引用文献

Fang, W.,Sanz, A.B.,Bartual, S.G.,Wang, B.,Ferenbach, A.T.,Farkas, V.,Hurtado-Guerrero, R.,Arroyo, J.,van Aalten, D.M.F.
Mechanisms of redundancy and specificity of the Aspergillus fumigatus Crh transglycosylases.
Nat Commun, 10:1669-1669, 2019

PubMed Abstract: Fungal cell wall synthesis is achieved by a balance of glycosyltransferase, hydrolase and transglycosylase activities. Transglycosylases strengthen the cell wall by forming a rigid network of crosslinks through mechanisms that remain to be explored. Here we study the function of the Aspergillus fumigatus family of five Crh transglycosylases. Although crh genes are dispensable for cell viability, simultaneous deletion of all genes renders cells sensitive to cell wall interfering compounds. In vitro biochemical assays and localisation studies demonstrate that this family of enzymes functions redundantly as transglycosylases for both chitin-glucan and chitin-chitin cell wall crosslinks. To understand the molecular basis of this acceptor promiscuity, we solved the crystal structure of A. fumigatus Crh5 (AfCrh5) in complex with a chitooligosaccharide at the resolution of 2.8 Å, revealing an extensive elongated binding cleft for the donor (-4 to -1) substrate and a short acceptor (+1 to +2) binding site. Together with mutagenesis, the structure suggests a "hydrolysis product assisted" molecular mechanism favouring transglycosylation over hydrolysis.

PubMed: 30971696
DOI: 10.1038/s41467-019-09674-0

実験手法

X-RAY DIFFRACTION (2.8 Å)