6IBP

Structure of a psychrophilic CCA-adding enzyme at room temperature in ChipX microfluidic device

6IBP の概要

• エントリーDOI: 10.2210/pdb6ibp/pdb
• 分子名称: CCA-adding enzyme (2 entities in total)
• 機能のキーワード: trna maturation, trna nucleotidyltransferase, chipx, psychrophilic enzyme, rna binding protein
• 由来する生物種: Planococcus halocryophilus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48498.27

構造登録者

de Wijn, R.,Hennig, O.,Rollet, K.,Bluhm, A.,Betat, H.,Moerl, M.,Lorber, B.,Sauter, C. (登録日: 2018-11-30, 公開日: 2019-05-29, 最終更新日: 2024-01-24)

主引用文献

de Wijn, R.,Hennig, O.,Roche, J.,Engilberge, S.,Rollet, K.,Fernandez-Millan, P.,Brillet, K.,Betat, H.,Morl, M.,Roussel, A.,Girard, E.,Mueller-Dieckmann, C.,Fox, G.C.,Olieric, V.,Gavira, J.A.,Lorber, B.,Sauter, C.
A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.
Iucrj, 6:454-464, 2019

PubMed Abstract: Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine 'non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.

PubMed: 31098026
DOI: 10.1107/S2052252519003622

実験手法

X-RAY DIFFRACTION (2.536 Å)