6IAW

Structure of head fiber and inner core protein gp22 of native bacteriophage P68

6IAW の概要

• エントリーDOI: 10.2210/pdb6iaw/pdb
• EMDBエントリー: 4440
• 分子名称: Major head protein, Arstotzka protein, Head fiber, ... (4 entities in total)
• 機能のキーワード: structural protein, bacteriophage, head fiber, inner core protein
• 由来する生物種: Staphylococcus phage P68; 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 350946.50

構造登録者

Hrebik, D.,Skubnik, K.,Fuzik, T.,Plevka, P. (登録日: 2018-11-27, 公開日: 2019-11-06, 最終更新日: 2024-05-15)

主引用文献

Hrebik, D.,Stverakova, D.,Skubnik, K.,Fuzik, T.,Pantucek, R.,Plevka, P.
Structure and genome ejection mechanism ofStaphylococcus aureusphage P68.
Sci Adv, 5:eaaw7414-eaaw7414, 2019

PubMed Abstract: Phages infecting can be used as therapeutics against antibiotic-resistant bacterial infections. However, there is limited information about the mechanism of genome delivery of phages that infect Gram-positive bacteria. Here, we present the structures of native phage P68, genome ejection intermediate, and empty particle. The P68 head contains 72 subunits of inner core protein, 15 of which bind to and alter the structure of adjacent major capsid proteins and thus specify attachment sites for head fibers. Unlike in the previously studied phages, the head fibers of P68 enable its virion to position itself at the cell surface for genome delivery. The unique interaction of one end of P68 DNA with one of the 12 portal protein subunits is disrupted before the genome ejection. The inner core proteins are released together with the DNA and enable the translocation of phage genome across the bacterial membrane into the cytoplasm.

PubMed: 31663016
DOI: 10.1126/sciadv.aaw7414

実験手法

ELECTRON MICROSCOPY (3.8 Å)