6IAK

The crystal structure of the chicken CREB3 bZIP

6IAK の概要

• エントリーDOI: 10.2210/pdb6iak/pdb
• 分子名称: Uncharacterized protein (1 entity in total)
• 機能のキーワード: creb3; bzip; crystallographic structure; homodimeric bzip unbound to dna., transcription
• 由来する生物種: Gallus gallus (Chicken)
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 341546.02

構造登録者

Sabaratnam, K.,Renner, M. (登録日: 2018-11-26, 公開日: 2019-12-11, 最終更新日: 2024-11-06)

主引用文献

Sabaratnam, K.,Renner, M.,Paesen, G.,Harlos, K.,Nair, V.,Owens, R.J.,Grimes, J.M.
Insights from the crystal structure of the chicken CREB3 bZIP suggest that members of the CREB3 subfamily transcription factors may be activated in response to oxidative stress.
Protein Sci., 28:779-787, 2019

PubMed Abstract: cAMP response element binding Protein 3 (CREB3) is an endoplasmic reticulum (ER) membrane-bound transcription factor, which belongs to the basic leucine zipper (bZIP) superfamily of eukaryotic transcription factors. CREB3 plays a role in the ER-stress induced unfolded protein response (UPR) and is a multifunctional cellular factor implicated in a number of biological processes including cell proliferation and migration, tumor suppression, and immune-related gene expression. To gain structural insights into the transcription factor, we determined the crystal structure of the conserved bZIP domain of chicken CREB3 (chCREB3) to a resolution of 3.95 Å. The X-ray structure provides evidence that chCREB3 can form a stable homodimer. The chCREB3 bZIP has a structured, pre-formed DNA binding region, even in the absence of DNA, a feature that could potentially enhance both the DNA binding specificity and affinity of chCREB3. Significantly, the homodimeric bZIP possesses an intermolecular disulfide bond that connects equivalent cysteine residues of the parallel helices in the leucine zipper region. This disulfide bond in the hydrophobic core of the bZIP may increase the stability of the homodimer under oxidizing conditions. Moreover, sequence alignment of bZIP sequences from chicken, human, and mouse reveals that only members of the CREB3 subfamily contain this cysteine residue, indicating that it could act as a redox-sensor. Taken together, these results suggest that the activity of these transcription factors may be redox-regulated and they may be activated in response to oxidative stress.

PubMed: 30653278
DOI: 10.1002/pro.3573

実験手法

X-RAY DIFFRACTION (3.95 Å)