6I98

Structure of the ferrioxamine B transporter FoxA from Pseudomonas aeruginosa, apo state

6I98 の概要

• エントリーDOI: 10.2210/pdb6i98/pdb
• 分子名称: Metal-pseudopaline receptor CntO, NICKEL (II) ION, GLYCEROL, ... (8 entities in total)
• 機能のキーワード: membrane protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 79006.53

構造登録者

Josts, I.,Tidow, H. (登録日: 2018-11-22, 公開日: 2019-08-28, 最終更新日: 2026-01-21)

主引用文献

Josts, I.,Veith, K.,Tidow, H.
Ternary structure of the outer membrane transporter FoxA with resolved signalling domain provides insights into TonB-mediated siderophore uptake.
Elife, 8:-, 2019

PubMed Abstract: Many microbes and fungi acquire the essential ion Fe through the synthesis and secretion of high-affinity chelators termed siderophores. In Gram-negative bacteria, these ferric-siderophore complexes are actively taken up using highly specific TonB-dependent transporters (TBDTs) located in the outer bacterial membrane (OM). However, the detailed mechanism of how the inner-membrane protein TonB connects to the transporters in the OM as well as the interplay between siderophore- and TonB-binding to the transporter is still poorly understood. Here, we present three crystal structures of the TBDT FoxA from (containing a signalling domain) in complex with the siderophore ferrioxamine B and TonB and combine them with a detailed analysis of binding constants. The structures show that both siderophore and TonB-binding is required to form a translocation-competent state of the FoxA transporter in a two-step TonB-binding mechanism. The complex structure also indicates how TonB-binding influences the orientation of the signalling domain.

PubMed: 31385808
DOI: 10.7554/eLife.48528

実験手法

X-RAY DIFFRACTION (2.8 Å)