6I96

Structure of the ferrioxamine B transporter FoxA from Pseudomonas aeruginosa in complex with ferrioxamine B

6I96 の概要

• エントリーDOI: 10.2210/pdb6i96/pdb
• 分子名称: Metal-pseudopaline receptor CntO, Ferrioxamine B, nonyl beta-D-glucopyranoside, ... (7 entities in total)
• 機能のキーワード: outer membrane iron, membrane protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 81185.49

構造登録者

Josts, I.,Tidow, H. (登録日: 2018-11-22, 公開日: 2019-08-28, 最終更新日: 2026-01-21)

主引用文献

Josts, I.,Veith, K.,Tidow, H.
Ternary structure of the outer membrane transporter FoxA with resolved signalling domain provides insights into TonB-mediated siderophore uptake.
Elife, 8:-, 2019

PubMed Abstract: Many microbes and fungi acquire the essential ion Fe through the synthesis and secretion of high-affinity chelators termed siderophores. In Gram-negative bacteria, these ferric-siderophore complexes are actively taken up using highly specific TonB-dependent transporters (TBDTs) located in the outer bacterial membrane (OM). However, the detailed mechanism of how the inner-membrane protein TonB connects to the transporters in the OM as well as the interplay between siderophore- and TonB-binding to the transporter is still poorly understood. Here, we present three crystal structures of the TBDT FoxA from (containing a signalling domain) in complex with the siderophore ferrioxamine B and TonB and combine them with a detailed analysis of binding constants. The structures show that both siderophore and TonB-binding is required to form a translocation-competent state of the FoxA transporter in a two-step TonB-binding mechanism. The complex structure also indicates how TonB-binding influences the orientation of the signalling domain.

PubMed: 31385808
DOI: 10.7554/eLife.48528

実験手法

X-RAY DIFFRACTION (1.85 Å)