6I86

Crocagin biosynthetic gene J

6I86 の概要

• エントリーDOI: 10.2210/pdb6i86/pdb
• 分子名称: Uncharacterized protein, GLYCEROL (3 entities in total)
• 機能のキーワード: cgnj, biosynthetic protein, domain of unknown function, crocagin, ripps
• 由来する生物種: Chondromyces crocatus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 52769.91

構造登録者

Adam, S.,Koehnke, J. (登録日: 2018-11-19, 公開日: 2019-03-13, 最終更新日: 2024-05-15)

主引用文献

Adam, S.,Klein, A.,Surup, F.,Koehnke, J.
The structure of CgnJ, a domain of unknown function protein from the crocagin gene cluster.
Acta Crystallogr.,Sect.F, 75:205-211, 2019

PubMed Abstract: Natural products often contain interesting new chemical entities that are introduced into the structure of a compound by the enzymatic machinery of the producing organism. The recently described crocagins are novel polycyclic peptides which belong to the class of ribosomally synthesized and post-translationally modified peptide natural products. They have been shown to bind to the conserved prokaryotic carbon-storage regulator A in vitro. In efforts to understand crocagin biosynthesis, the putative biosynthetic genes were expressed and purified. Here, the first crystal structure of a protein from the crocagin-biosynthetic gene cluster, CgnJ, a domain of unknown function protein, is reported. Possible functions of this protein were explored by structural and sequence homology analyses. Even though the sequence homology to proteins in the Protein Data Bank is low, the protein shows significant structural homology to a protein with known function within the competency system of Bacillus subtilis, ComJ, leading to the hypothesis of a similar role of the protein within the producing organism.

PubMed: 30839296
DOI: 10.1107/S2053230X19000712

実験手法

X-RAY DIFFRACTION (2 Å)