Summary for 6I5C
| Entry DOI | 10.2210/pdb6i5c/pdb |
| Related | 4WBN |
| Descriptor | Tubulin alpha-1B chain, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER, ... (12 entities in total) |
| Functional Keywords | tubulin complex, t2r-ttl, long-wavelength native-sad phasing, native-sad, s-sad, structural protein |
| Biological source | Bos taurus (Bovine) More |
| Total number of polymer chains | 6 |
| Total formula weight | 257658.23 |
| Authors | Basu, S.,Olieric, V.,Matsugaki, N.,Kawano, Y.,Takashi, T.,Huang, C.Y.,Leonarski, F.,Yamada, Y.,Vera, L.,Olieric, N.,Basquin, J.,Wojdyla, J.A.,Diederichs, K.,Yamamoto, M.,Bunk, O.,Wang, M. (deposition date: 2018-11-13, release date: 2019-03-13, Last modification date: 2024-05-15) |
| Primary citation | Basu, S.,Olieric, V.,Leonarski, F.,Matsugaki, N.,Kawano, Y.,Takashi, T.,Huang, C.Y.,Yamada, Y.,Vera, L.,Olieric, N.,Basquin, J.,Wojdyla, J.A.,Bunk, O.,Diederichs, K.,Yamamoto, M.,Wang, M. Long-wavelength native-SAD phasing: opportunities and challenges. Iucrj, 6:373-386, 2019 Cited by PubMed Abstract: Native single-wavelength anomalous dispersion (SAD) is an attractive experimental phasing technique as it exploits weak anomalous signals from intrinsic light scatterers ( < 20). The anomalous signal of sulfur in particular, is enhanced at long wavelengths, however the absorption of diffracted X-rays owing to the crystal, the sample support and air affects the recorded intensities. Thereby, the optimal measurable anomalous signals primarily depend on the counterplay of the absorption and the anomalous scattering factor at a given X-ray wavelength. Here, the benefit of using a wavelength of 2.7 over 1.9 Å is demonstrated for native-SAD phasing on a 266 kDa multiprotein-ligand tubulin complex (TR-TTL) and is applied in the structure determination of an 86 kDa helicase Sen1 protein at beamline BL-1A of the KEK Photon Factory, Japan. Furthermore, X-ray absorption at long wavelengths was controlled by shaping a lysozyme crystal into spheres of defined thicknesses using a deep-UV laser, and a systematic comparison between wavelengths of 2.7 and 3.3 Å is reported for native SAD. The potential of laser-shaping technology and other challenges for an optimized native-SAD experiment at wavelengths >3 Å are discussed. PubMed: 31098019DOI: 10.1107/S2052252519002756 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.95 Å) |
Structure validation
Download full validation report
