6HXA

AntI from P. luminescens catalyses terminal polyketide shortening in the biosynthesis of anthraquinones

6HXA の概要

• エントリーDOI: 10.2210/pdb6hxa/pdb
• 分子名称: AntI, TRIETHYLENE GLYCOL, TETRAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: natural product biosynthesis, type ii polyketide synthase system, lyase, alpha, beta-hydrolase fold, cascade reaction, retro claisen reaction, dieckmann condensation
• 由来する生物種: Photorhabdus luminescens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46125.54

構造登録者

Zhou, Q.,Braeuer, A.,Grammbitter, G.,Schmalhofer, M.,Saura, P.,Adihou, H.,Kaila, V.R.I.,Groll, M.,Bode, H. (登録日: 2018-10-16, 公開日: 2019-08-07, 最終更新日: 2024-05-01)

主引用文献

Zhou, Q.,Brauer, A.,Adihou, H.,Schmalhofer, M.,Saura, P.,Grammbitter, G.L.C.,Kaila, V.R.I.,Groll, M.,Bode, H.B.
Molecular mechanism of polyketide shortening in anthraquinone biosynthesis ofPhotorhabdus luminescens.
Chem Sci, 10:6341-6349, 2019

PubMed Abstract: Anthraquinones, a widely distributed class of aromatic natural products, are produced by a type II polyketide synthase system in the Gram-negative bacterium . Heterologous expression of the anthraquinone biosynthetic gene cluster in identified AntI as an unusual lyase, catalysing terminal polyketide shortening prior to formation of the third aromatic ring. Functional and analysis of AntI using X-ray crystallography, structure-based mutagenesis, and molecular simulations revealed that AntI converts a defined octaketide to the tricyclic anthraquinone ring retro-Claisen and Dieckmann reactions. Thus, AntI catalyses a so far unobserved multistep reaction in this PKS system.

PubMed: 31341589
DOI: 10.1039/c9sc00749k

実験手法

X-RAY DIFFRACTION (1.85 Å)