6HVO

Crystal structure of human PCNA in complex with three peptides of p12 subunit of human polymerase delta

6HVO の概要

• エントリーDOI: 10.2210/pdb6hvo/pdb
• 分子名称: Proliferating cell nuclear antigen, DNA polymerase delta subunit 4, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: pcna, pold4, p12, replication, polymerase delta, crystal
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 94739.72

構造登録者

Gonzalez-Magana, A.,Romano-Moreno, M.,Rojas, A.L.,Blanco, F.J.,De Biasio, A. (登録日: 2018-10-11, 公開日: 2019-01-23, 最終更新日: 2019-03-27)

主引用文献

Gonzalez-Magana, A.,Ibanez de Opakua, A.,Romano-Moreno, M.,Murciano-Calles, J.,Merino, N.,Luque, I.,Rojas, A.L.,Onesti, S.,Blanco, F.J.,De Biasio, A.
The p12 subunit of human polymerase delta uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA).
J.Biol.Chem., 294:3947-3956, 2019

PubMed Abstract: Human DNA polymerase δ is essential for DNA replication and acts in conjunction with the processivity factor proliferating cell nuclear antigen (PCNA). In addition to its catalytic subunit (p125), pol δ comprises three regulatory subunits (p50, p68, and p12). PCNA interacts with all of these subunits, but only the interaction with p68 has been structurally characterized. Here, we report solution NMR-, isothermal calorimetry-, and X-ray crystallography-based analyses of the p12-PCNA interaction, which takes part in the modulation of the rate and fidelity of DNA synthesis by pol δ. We show that p12 binds with micromolar affinity to the classical PIP-binding pocket of PCNA via a highly atypical PIP box located at the p12 N terminus. Unlike the canonical PIP box of p68, the PIP box of p12 lacks the conserved glutamine; binds through a 2-fork plug made of an isoleucine and a tyrosine residue at +3 and +8 positions, respectively; and is stabilized by an aspartate at +6 position, which creates a network of intramolecular hydrogen bonds. These findings add to growing evidence that PCNA can bind a diverse range of protein sequences that may be broadly grouped as PIP-like motifs as has been previously suggested.

PubMed: 30655288
DOI: 10.1074/jbc.RA118.006391

実験手法

X-RAY DIFFRACTION (2.1 Å)