6HUS

2'-fucosyllactose and 3-fucosyllactose binding protein from Bifidobacterium longum infantis, bound with 3-fucosyllactose

Summary for 6HUS

• Entry DOI: 10.2210/pdb6hus/pdb
• Descriptor: ABC transporter substrate-binding protein, beta-D-galactopyranose-(1-4)-beta-D-glucopyranose-(1-3)-alpha-L-fucopyranose-(1-3)-alpha-D-glucopyranose, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (5 entities in total)
• Functional Keywords: solute binding protein, 2'-fucosyllactose, bifidobacterium longum infantis, abc-transporter, transport protein
• Biological source: Bifidobacterium longum subsp. infantis
• Total number of polymer chains: 1
• Total formula weight: 49296.15

Authors

Ejby, M.,Abou Hachem, M.,Lo Leggio, L.,Takane, K.,Sakanaka, M. (deposition date: 2018-10-09, release date: 2019-09-04, Last modification date: 2024-05-15)

Primary citation

Sakanaka, M.,Hansen, M.E.,Gotoh, A.,Katoh, T.,Yoshida, K.,Odamaki, T.,Yachi, H.,Sugiyama, Y.,Kurihara, S.,Hirose, J.,Urashima, T.,Xiao, J.Z.,Kitaoka, M.,Fukiya, S.,Yokota, A.,Lo Leggio, L.,Abou Hachem, M.,Katayama, T.
Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis.
Sci Adv, 5:eaaw7696-eaaw7696, 2019

PubMed Abstract: The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that human milk oligosaccharides (HMOs) in breast milk promote the formation of a bifidobacteria-rich microbiota in infant guts; however, the underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from subspecies . Fecal DNA and HMO consumption analyses, combined with deposited metagenome data mining, revealed that FL transporter-2 is primarily associated with the bifidobacteria-rich microbiota formation in breast-fed infant guts. Structural analyses of the solute-binding protein (SBP) of FL transporter-2 complexed with 2'-fucosyllactose and 3-fucosyllactose, together with phylogenetic analysis of SBP homologs of both FL transporters, highlight a unique adaptation strategy of to HMOs, in which the gain-of-function mutations enable FL transporter-2 to efficiently capture major fucosylated HMOs. Our results provide a molecular insight into HMO-mediated symbiosis and coevolution between bifidobacteria and humans.

PubMed: 31489370
DOI: 10.1126/sciadv.aaw7696

Experimental method

X-RAY DIFFRACTION (1.409 Å)