6HRG

Structure of Igni18, a novel metallo hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I

6HRG の概要

• エントリーDOI: 10.2210/pdb6hrg/pdb
• 分子名称: UPF0173 metal-dependent hydrolase Igni_1254, ZINC ION, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: ignococcus hospitalis, hydrolase, metallo-b-lactamase
• 由来する生物種: Ignicoccus hospitalis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28972.63

構造登録者

Smits, S.H.,Streit, W.R.,Jaeger, K.E.,Hoeppner, A. (登録日: 2018-09-26, 公開日: 2019-10-09, 最終更新日: 2024-05-15)

主引用文献

Perez-Garcia, P.,Kobus, S.,Gertzen, C.G.W.,Hoeppner, A.,Holzscheck, N.,Strunk, C.H.,Huber, H.,Jaeger, K.E.,Gohlke, H.,Kovacic, F.,Smits, S.H.J.,Streit, W.R.,Chow, J.
A promiscuous ancestral enzyme ́s structure unveils protein variable regions of the highly diverse metallo-beta-lactamase family.
Commun Biol, 4:132-132, 2021

PubMed Abstract: The metallo-β-lactamase fold is an ancient protein structure present in numerous enzyme families responsible for diverse biological processes. The crystal structure of the hyperthermostable crenarchaeal enzyme Igni18 from Ignicoccus hospitalis was solved at 2.3 Å and could resemble a possible first archetype of a multifunctional metallo-β-lactamase. Ancestral enzymes at the evolutionary origin are believed to be promiscuous all-rounders. Consistently, Igni18´s activity can be cofactor-dependently directed from β-lactamase to lactonase, lipase, phosphodiesterase, phosphotriesterase or phospholipase. Its core-domain is highly conserved within metallo-β-lactamases from Bacteria, Archaea and Eukarya and gives insights into evolution and function of enzymes from this superfamily. Structural alignments with diverse metallo-β-lactamase-fold-containing enzymes allowed the identification of Protein Variable Regions accounting for modulation of activity, specificity and oligomerization patterns. Docking of different substrates within the active sites revealed the basis for the crucial cofactor dependency of this enzyme superfamily.

PubMed: 33514861
DOI: 10.1038/s42003-021-01671-8

実験手法

X-RAY DIFFRACTION (2.12 Å)