6HNJ
The ligand-bound, open structure of CD0873, a substrate binding protein with adhesive properties from Clostridium difficile.
Summary for 6HNJ
| Entry DOI | 10.2210/pdb6hnj/pdb |
| Descriptor | ABC-type transport system, sugar-family extracellular solute-binding protein, 1,2-ETHANEDIOL, TRIETHYLENE GLYCOL, ... (5 entities in total) |
| Functional Keywords | substrate binding protein, adhesin, tyrosine binding protein, lipoprotein, transport protein |
| Biological source | Peptoclostridium difficile 630 (Clostridium difficile) |
| Total number of polymer chains | 1 |
| Total formula weight | 34533.99 |
| Authors | Bradshaw, W.J.,Kovacs-Simon, A.,Harmer, N.J.,Michell, S.L.,Acharya, K.R. (deposition date: 2018-09-15, release date: 2019-08-28, Last modification date: 2024-05-15) |
| Primary citation | Bradshaw, W.J.,Bruxelle, J.F.,Kovacs-Simon, A.,Harmer, N.J.,Janoir, C.,Pechine, S.,Acharya, K.R.,Michell, S.L. Molecular features of lipoprotein CD0873: A potential vaccine against the human pathogenClostridioides difficile. J.Biol.Chem., 294:15850-15861, 2019 Cited by PubMed Abstract: is the primary cause of antibiotic-associated diarrhea and colitis, a healthcare-associated intestinal disease resulting in a significant fatality rate. Colonization of the gut is critical for pathogenesis. The bacterial molecules essential for efficient colonization therefore offer great potential as vaccine candidates. Here we present findings demonstrating that the immunogenic lipoprotein CD0873 plays a critical role in pathogen success We found that in a dixenic colonization model, a CD0873-positive strain of significantly outcompeted a CD0873-negative strain. Immunization of mice with recombinant CD0873 prevented long-term gut colonization and was correlated with a strong secretory IgA immune response. We further present high-resolution crystal structures of CD0873, at 1.35-2.50 Å resolutions, offering a first view of the ligand-binding pocket of CD0873 and provide evidence that this lipoprotein adhesin is part of a tyrosine import system, an amino acid key in infection. These findings suggest that CD0873 could serve as an effective component in a vaccine against . PubMed: 31420448DOI: 10.1074/jbc.RA119.010120 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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