6HKG

Structure of FISW84 Fab Fragment

6HKG の概要

• エントリーDOI: 10.2210/pdb6hkg/pdb
• 分子名称: FISW84 Fab Fragment Heavy Chain, FISW84 Fab Fragment Light Chain, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 84916.74

構造登録者

Benton, D.J.,Rosenthal, P.B. (登録日: 2018-09-06, 公開日: 2018-09-26, 最終更新日: 2024-10-09)

主引用文献

Benton, D.J.,Nans, A.,Calder, L.J.,Turner, J.,Neu, U.,Lin, Y.P.,Ketelaars, E.,Kallewaard, N.L.,Corti, D.,Lanzavecchia, A.,Gamblin, S.J.,Rosenthal, P.B.,Skehel, J.J.
Influenza hemagglutinin membrane anchor.
Proc. Natl. Acad. Sci. U.S.A., 115:10112-10117, 2018

PubMed Abstract: Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the hemagglutinin (HA), the 3D structure of which is known from X-ray crystallographic studies. The soluble ectodomain fragments used in these studies lacked the "membrane anchor" portion of the molecule. Since this region has a role in membrane fusion, we have determined its structure by analyzing the intact, full-length molecule in a detergent micelle, using cryo-EM. We have also compared the structures of full-length HA-detergent micelles with full-length HA-Fab complex detergent micelles, to describe an infectivity-neutralizing monoclonal Fab that binds near the ectodomain membrane anchor junction. We determine a high-resolution HA structure which compares favorably in detail with the structure of the ectodomain seen by X-ray crystallography; we detect, clearly, all five carbohydrate side chains of HA; and we find that the ectodomain is joined to the membrane anchor by flexible, eight-residue-long, linkers. The linkers extend into the detergent micelle to join a central triple-helical structure that is a major component of the membrane anchor.

PubMed: 30224494
DOI: 10.1073/pnas.1810927115

実験手法

X-RAY DIFFRACTION (1.87 Å)