6HJN
Structure of Influenza Hemagglutinin ectodomain (A/duck/Alberta/35/76)
Summary for 6HJN
| Entry DOI | 10.2210/pdb6hjn/pdb |
| EMDB information | 0234 |
| Descriptor | Hemagglutinin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total) |
| Functional Keywords | influenza virus, hemagglutinin, membrane protein, membrane fusion, viral protein |
| Biological source | Influenza A virus (strain A/Duck/Alberta/35/1976 H1N1) More |
| Total number of polymer chains | 6 |
| Total formula weight | 176418.47 |
| Authors | Benton, D.J.,Rosenthal, P.B. (deposition date: 2018-09-04, release date: 2018-09-26, Last modification date: 2024-10-16) |
| Primary citation | Benton, D.J.,Nans, A.,Calder, L.J.,Turner, J.,Neu, U.,Lin, Y.P.,Ketelaars, E.,Kallewaard, N.L.,Corti, D.,Lanzavecchia, A.,Gamblin, S.J.,Rosenthal, P.B.,Skehel, J.J. Influenza hemagglutinin membrane anchor. Proc. Natl. Acad. Sci. U.S.A., 115:10112-10117, 2018 Cited by PubMed Abstract: Viruses with membranes fuse them with cellular membranes, to transfer their genomes into cells at the beginning of infection. For Influenza virus, the membrane glycoprotein involved in fusion is the hemagglutinin (HA), the 3D structure of which is known from X-ray crystallographic studies. The soluble ectodomain fragments used in these studies lacked the "membrane anchor" portion of the molecule. Since this region has a role in membrane fusion, we have determined its structure by analyzing the intact, full-length molecule in a detergent micelle, using cryo-EM. We have also compared the structures of full-length HA-detergent micelles with full-length HA-Fab complex detergent micelles, to describe an infectivity-neutralizing monoclonal Fab that binds near the ectodomain membrane anchor junction. We determine a high-resolution HA structure which compares favorably in detail with the structure of the ectodomain seen by X-ray crystallography; we detect, clearly, all five carbohydrate side chains of HA; and we find that the ectodomain is joined to the membrane anchor by flexible, eight-residue-long, linkers. The linkers extend into the detergent micelle to join a central triple-helical structure that is a major component of the membrane anchor. PubMed: 30224494DOI: 10.1073/pnas.1810927115 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.3 Å) |
Structure validation
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