6HHM

Crystal structure of the family S1_7 ulvan-specific sulfatase FA22070 from Formosa agariphila

6HHM の概要

• エントリーDOI: 10.2210/pdb6hhm/pdb
• 分子名称: Arylsulfatase, CALCIUM ION (3 entities in total)
• 機能のキーワード: formosa agariphila algal polysaccharide carrageenan sulfatase, hydrolase
• 由来する生物種: Formosa agariphila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 53968.80

構造登録者

Roret, T.,Prechoux, A.,Michel, G.,Czjzek, M. (登録日: 2018-08-28, 公開日: 2019-06-26, 最終更新日: 2024-01-17)

主引用文献

Reisky, L.,Prechoux, A.,Zuhlke, M.K.,Baumgen, M.,Robb, C.S.,Gerlach, N.,Roret, T.,Stanetty, C.,Larocque, R.,Michel, G.,Song, T.,Markert, S.,Unfried, F.,Mihovilovic, M.D.,Trautwein-Schult, A.,Becher, D.,Schweder, T.,Bornscheuer, U.T.,Hehemann, J.H.
A marine bacterial enzymatic cascade degrades the algal polysaccharide ulvan.
Nat.Chem.Biol., 15:803-812, 2019

PubMed Abstract: Marine seaweeds increasingly grow into extensive algal blooms, which are detrimental to coastal ecosystems, tourism and aquaculture. However, algal biomass is also emerging as a sustainable raw material for the bioeconomy. The potential exploitation of algae is hindered by our limited knowledge of the microbial pathways-and hence the distinct biochemical functions of the enzymes involved-that convert algal polysaccharides into oligo- and monosaccharides. Understanding these processes would be essential, however, for applications such as the fermentation of algal biomass into bioethanol or other value-added compounds. Here, we describe the metabolic pathway that enables the marine flavobacterium Formosa agariphila to degrade ulvan, the main cell wall polysaccharide of bloom-forming Ulva species. The pathway involves 12 biochemically characterized carbohydrate-active enzymes, including two polysaccharide lyases, three sulfatases and seven glycoside hydrolases that sequentially break down ulvan into fermentable monosaccharides. This way, the enzymes turn a previously unexploited renewable into a valuable and ecologically sustainable bioresource.

PubMed: 31285597
DOI: 10.1038/s41589-019-0311-9

実験手法

X-RAY DIFFRACTION (1.23 Å)