6HCT

Crystal structure of Archeoglobus fulgidus L7Ae bound to its cognate UTR k-turn

Summary for 6HCT

• Entry DOI: 10.2210/pdb6hct/pdb
• Descriptor: RNA (5'-R(*GP*CP*CP*GP*AP*UP*GP*AP*AP*UP*GP*CP*AP*UP*GP*AP*AP*GP*C)-3'), 50S ribosomal protein L7Ae, RNA (5'-R(*GP*CP*CP*GP*AP*UP*GP*AP*AP*UP*G)-3'), ... (6 entities in total)
• Functional Keywords: gene regulation; rna structure; kink-turn; x-ray crystallography, rna
• Biological source: Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126); More
• Total number of polymer chains: 7
• Total formula weight: 57297.96

Authors

Huang, L.,Lilley, D.M.J. (deposition date: 2018-08-16, release date: 2018-10-31, Last modification date: 2024-01-17)

Primary citation

Huang, L.,Ashraf, S.,Lilley, D.M.J.
The role of RNA structure in translational regulation by L7Ae protein in archaea.
RNA, 25:60-69, 2019

PubMed Abstract: A recent study has shown that archaeal L7Ae binds to a putative k-turn structure in the 5'-leader of the mRNA of its structural gene to regulate translation. To function as a regulator, the RNA should be unstructured in the absence of protein, but it should adopt a k-turn-containing stem-loop on binding L7Ae. Sequence analysis of UTR sequences indicates that their k-turn elements will be unable to fold in the absence of L7Ae, and we have demonstrated this experimentally in solution using FRET for the sequence. We have solved the X-ray crystal structure of the complex of the RNA bound to its cognate L7Ae protein. The RNA adopts a standard k-turn conformation that is specifically recognized by the L7Ae protein, so stabilizing the stem-loop. In-line probing of the natural-sequence UTR shows that the RNA is unstructured in the absence of L7Ae binding, but folds on binding the protein such that the ribosome binding site is occluded. Thus, L7Ae regulates its own translation by switching the conformation of the RNA to alter accessibility.

PubMed: 30327333
DOI: 10.1261/rna.068510.118

Experimental method

X-RAY DIFFRACTION (3.091 Å)