6HCQ

Structure of the rabbit collided di-ribosome (collided monosome)

これはPDB形式変換不可エントリーです。

6HCQ の概要

• エントリーDOI: 10.2210/pdb6hcq/pdb
• EMDBエントリー: 0197
• 分子名称: 28S ribosomal RNA, Ribosomal protein S5, 40S ribosomal protein S6, ... (87 entities in total)
• 機能のキーワード: translation, quality control, ribosome
• 由来する生物種: Oryctolagus cuniculus; 詳細
• タンパク質・核酸の鎖数: 85
• 化学式量合計: 3462706.10

構造登録者

Juszkiewicz, S.,Chandrasekaran, V.,Lin, Z.,Kraatz, S.,Ramakrishnan, V.,Hegde, R.S. (登録日: 2018-08-16, 公開日: 2018-10-17, 最終更新日: 2018-11-14)

主引用文献

Juszkiewicz, S.,Chandrasekaran, V.,Lin, Z.,Kraatz, S.,Ramakrishnan, V.,Hegde, R.S.
ZNF598 Is a Quality Control Sensor of Collided Ribosomes.
Mol. Cell, 72:469-481.e7, 2018

PubMed Abstract: Aberrantly slow translation elicits quality control pathways initiated by the ubiquitin ligase ZNF598. How ZNF598 discriminates physiologic from pathologic translation complexes and ubiquitinates stalled ribosomes selectively is unclear. Here, we find that the minimal unit engaged by ZNF598 is the collided di-ribosome, a molecular species that arises when a trailing ribosome encounters a slower leading ribosome. The collided di-ribosome structure reveals an extensive 40S-40S interface in which the ubiquitination targets of ZNF598 reside. The paucity of 60S interactions allows for different ribosome rotation states, explaining why ZNF598 recognition is indifferent to how the leading ribosome has stalled. The use of ribosome collisions as a proxy for stalling allows the degree of tolerable slowdown to be tuned by the initiation rate on that mRNA; hence, the threshold for triggering quality control is substrate specific. These findings illustrate how higher-order ribosome architecture can be exploited by cellular factors to monitor translation status.

PubMed: 30293783
DOI: 10.1016/j.molcel.2018.08.037

実験手法

ELECTRON MICROSCOPY (6.5 Å)