6H97

AlbAT99V mutant , albicidin resistance protein

6H97 の概要

• エントリーDOI: 10.2210/pdb6h97/pdb
• 分子名称: Albicidin resistance protein (2 entities in total)
• 機能のキーワード: albicidin resistance protein, albicidin binding protein
• 由来する生物種: Klebsiella oxytoca
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26479.94

構造登録者

Koehnke, J.,Sikandar, A. (登録日: 2018-08-03, 公開日: 2018-11-21, 最終更新日: 2024-05-15)

主引用文献

Sikandar, A.,Cirnski, K.,Testolin, G.,Volz, C.,Bronstrup, M.,Kalinina, O.V.,Muller, R.,Koehnke, J.
Adaptation of a Bacterial Multidrug Resistance System Revealed by the Structure and Function of AlbA.
J.Am.Chem.Soc., 140:16641-16649, 2018

PubMed Abstract: To combat the rise of antimicrobial resistance, the discovery of new antibiotics is paramount. Albicidin and cystobactamid are related natural product antibiotics with potent activity against Gram-positive and, crucially, Gram-negative pathogens. AlbA has been reported to neutralize albicidin by binding it with nanomolar affinity. To understand this potential resistance mechanism, we determined structures of AlbA and its complex with albicidin. The structures revealed AlbA to be comprised of two domains, each unexpectedly resembling the multiantibiotic neutralizing protein TipA. Binding of the long albicidin molecule was shared pseudosymmetrically between the two domains. The structure also revealed an unexpected chemical modification of albicidin, which we demonstrate to be promoted by AlbA, and to reduce albicidin potency; we propose a mechanism for this reaction. Overall, our findings suggest that AlbA arose through internal duplication in an ancient TipA-like gene, leading to a new binding scaffold adapted to the sequestration of long-chain antibiotics.

PubMed: 30422653
DOI: 10.1021/jacs.8b08895

実験手法

X-RAY DIFFRACTION (2.598 Å)