6H7W

Model of retromer-Vps5 complex assembled on membrane.

6H7W の概要

• エントリーDOI: 10.2210/pdb6h7w/pdb
• EMDBエントリー: 0154
• 分子名称: Vacuolar protein sorting-associated protein 26-like protein, Putative vacuolar protein sorting-associated protein, Vacuolar protein sorting-associated protein 29, ... (6 entities in total)
• 機能のキーワード: retromer, endosome, sorting nexin, bar, membrane trafficking, protein transport
• 由来する生物種: Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719); 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 750872.07

構造登録者

Kovtun, O.,Leneva, N.,Ariotti, N.,Rohan, T.S.,Owen, D.J.,Briggs, J.A.G.,Collins, B.M. (登録日: 2018-07-31, 公開日: 2018-09-26, 最終更新日: 2024-05-15)

主引用文献

Kovtun, O.,Leneva, N.,Bykov, Y.S.,Ariotti, N.,Teasdale, R.D.,Schaffer, M.,Engel, B.D.,Owen, D.J.,Briggs, J.A.G.,Collins, B.M.
Structure of the membrane-assembled retromer coat determined by cryo-electron tomography.
Nature, 561:561-564, 2018

PubMed Abstract: Eukaryotic cells traffic proteins and lipids between different compartments using protein-coated vesicles and tubules. The retromer complex is required to generate cargo-selective tubulovesicular carriers from endosomal membranes. Conserved in eukaryotes, retromer controls the cellular localization and homeostasis of hundreds of transmembrane proteins, and its disruption is associated with major neurodegenerative disorders. How retromer is assembled and how it is recruited to form coated tubules is not known. Here we describe the structure of the retromer complex (Vps26-Vps29-Vps35) assembled on membrane tubules with the bin/amphiphysin/rvs-domain-containing sorting nexin protein Vps5, using cryo-electron tomography and subtomogram averaging. This reveals a membrane-associated Vps5 array, from which arches of retromer extend away from the membrane surface. Vps35 forms the 'legs' of these arches, and Vps29 resides at the apex where it is free to interact with regulatory factors. The bases of the arches connect to each other and to Vps5 through Vps26, and the presence of the same arches on coated tubules within cells confirms their functional importance. Vps5 binds to Vps26 at a position analogous to the previously described cargo- and Snx3-binding site, which suggests the existence of distinct retromer-sorting nexin assemblies. The structure provides insight into the architecture of the coat and its mechanism of assembly, and suggests that retromer promotes tubule formation by directing the distribution of sorting nexin proteins on the membrane surface while providing a scaffold for regulatory-protein interactions.

PubMed: 30224749
DOI: 10.1038/s41586-018-0526-z

実験手法

ELECTRON MICROSCOPY (11.4 Å)