6H7V

Crystal structure of BauA, the Ferric preacinetobactin receptor from Acinetobacter baumannii

6H7V の概要

• エントリーDOI: 10.2210/pdb6h7v/pdb
• 関連するPDBエントリー: 6H7F
• 分子名称: Ligand-gated channel protein, 1,2-ETHANEDIOL, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE, ... (4 entities in total)
• 機能のキーワード: baua, outer-membrane transporter, membrane protein
• 由来する生物種: Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / CIP 70.34 / JCM 6841 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 235161.29

構造登録者

Moynie, L.,Naismith, J.H. (登録日: 2018-07-31, 公開日: 2018-10-10, 最終更新日: 2024-10-23)

主引用文献

Moynie, L.,Serra, I.,Scorciapino, M.A.,Oueis, E.,Page, M.G.,Ceccarelli, M.,Naismith, J.H.
Preacinetobactin not acinetobactin is essential for iron uptake by the BauA transporter of the pathogenAcinetobacter baumannii.
Elife, 7:-, 2018

PubMed Abstract: New strategies are urgently required to develop antibiotics. The siderophore uptake system has attracted considerable attention, but rational design of siderophore antibiotic conjugates requires knowledge of recognition by the cognate outer-membrane transporter. is a serious pathogen, which utilizes (pre)acinetobactin to scavenge iron from the host. We report the structure of the (pre)acinetobactin transporter BauA bound to the siderophore, identifying the structural determinants of recognition. Detailed biophysical analysis confirms that BauA recognises preacinetobactin. We show that acinetobactin is not recognised by the protein, thus preacinetobactin is essential for iron uptake. The structure shows and NMR confirms that under physiological conditions, a molecule of acinetobactin will bind to two free coordination sites on the iron preacinetobactin complex. The ability to recognise a heterotrimeric iron-preacinetobactin-acinetobactin complex may rationalize contradictory reports in the literature. These results open new avenues for the design of novel antibiotic conjugates (trojan horse) antibiotics.

PubMed: 30558715
DOI: 10.7554/eLife.42270

実験手法

X-RAY DIFFRACTION (2.54 Å)