6H7F

Crystal structure of BauA, the Ferric preacinetobactin receptor from Acinetobacter baumannii in complex with Fe3+-Preacinetobactin-acinetobactin

6H7F の概要

• エントリーDOI: 10.2210/pdb6h7f/pdb
• 分子名称: BauA, FE (III) ION, (4~{S},5~{R})-2-[2,3-bis(oxidanyl)phenyl]-~{N}-[2-(1~{H}-imidazol-4-yl)ethyl]-5-methyl-~{N}-oxidanyl-4,5-dihydro-1,3-oxazole-4-carboxamide, ... (8 entities in total)
• 機能のキーワード: baua, outer-membrane transporter, membrane protein
• 由来する生物種: Acinetobacter baumannii
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 242880.61

構造登録者

Moynie, L.,Naismith, J.H. (登録日: 2018-07-31, 公開日: 2018-10-10, 最終更新日: 2024-01-17)

主引用文献

Moynie, L.,Serra, I.,Scorciapino, M.A.,Oueis, E.,Page, M.G.,Ceccarelli, M.,Naismith, J.H.
Preacinetobactin not acinetobactin is essential for iron uptake by the BauA transporter of the pathogenAcinetobacter baumannii.
Elife, 7:-, 2018

PubMed Abstract: New strategies are urgently required to develop antibiotics. The siderophore uptake system has attracted considerable attention, but rational design of siderophore antibiotic conjugates requires knowledge of recognition by the cognate outer-membrane transporter. is a serious pathogen, which utilizes (pre)acinetobactin to scavenge iron from the host. We report the structure of the (pre)acinetobactin transporter BauA bound to the siderophore, identifying the structural determinants of recognition. Detailed biophysical analysis confirms that BauA recognises preacinetobactin. We show that acinetobactin is not recognised by the protein, thus preacinetobactin is essential for iron uptake. The structure shows and NMR confirms that under physiological conditions, a molecule of acinetobactin will bind to two free coordination sites on the iron preacinetobactin complex. The ability to recognise a heterotrimeric iron-preacinetobactin-acinetobactin complex may rationalize contradictory reports in the literature. These results open new avenues for the design of novel antibiotic conjugates (trojan horse) antibiotics.

PubMed: 30558715
DOI: 10.7554/eLife.42270

実験手法

X-RAY DIFFRACTION (2.26 Å)