6H42

crystal structure of the human TGT catalytic subunit QTRT1

6H42 の概要

• エントリーDOI: 10.2210/pdb6h42/pdb
• 分子名称: Queuine tRNA-ribosyltransferase catalytic subunit 1, POTASSIUM ION, ZINC ION, ... (7 entities in total)
• 機能のキーワード: transglycosylase, queuine, trna, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 87261.65

構造登録者

Johannsson, S.,Neumann, P.,Ficner, R. (登録日: 2018-07-20, 公開日: 2018-09-05, 最終更新日: 2024-06-19)

主引用文献

Johannsson, S.,Neumann, P.,Ficner, R.
Crystal Structure of the Human tRNA Guanine Transglycosylase Catalytic Subunit QTRT1.
Biomolecules, 8:-, 2018

PubMed Abstract: RNA modifications have been implicated in diverse and important roles in all kingdoms of life with over 100 of them present on tRNAs. A prominent modification at the wobble base of four tRNAs is the 7-deaza-guanine derivative queuine which substitutes the guanine at position 34. This exchange is catalyzed by members of the enzyme class of tRNA guanine transglycosylases (TGTs). These enzymes incorporate guanine substituents into tRNA, tRNA tRNA, and tRNA in all kingdoms of life. In contrast to the homodimeric bacterial TGT, the active eukaryotic TGT is a heterodimer in solution, comprised of a catalytic QTRT1 subunit and a noncatalytic QTRT2 subunit. Bacterial TGT enzymes, that incorporate a queuine precursor, have been identified or proposed as virulence factors for infections by pathogens in humans and therefore are valuable targets for drug design. To date no structure of a eukaryotic catalytic subunit is reported, and differences to its bacterial counterpart have to be deducted from sequence analysis and models. Here we report the first crystal structure of a eukaryotic QTRT1 subunit and compare it to known structures of the bacterial TGT and murine QTRT2. Furthermore, we were able to determine the crystal structure of QTRT1 in complex with the queuine substrate.

PubMed: 30149595
DOI: 10.3390/biom8030081

実験手法

X-RAY DIFFRACTION (2.45 Å)