6H2X

MukB coiled-coil elbow from E. coli

6H2X の概要

• エントリーDOI: 10.2210/pdb6h2x/pdb
• 分子名称: Chromosome partition protein MukB,Chromosome partition protein MukB (2 entities in total)
• 機能のキーワード: chromosome organization, chromosome segregation, coiled coil, dna binding protein
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42356.71

構造登録者

Buermann, F.,Lowe, J. (登録日: 2018-07-16, 公開日: 2019-03-06, 最終更新日: 2024-10-23)

主引用文献

Burmann, F.,Lee, B.G.,Than, T.,Sinn, L.,O'Reilly, F.J.,Yatskevich, S.,Rappsilber, J.,Hu, B.,Nasmyth, K.,Lowe, J.
A folded conformation of MukBEF and cohesin.
Nat.Struct.Mol.Biol., 26:227-236, 2019

PubMed Abstract: Structural maintenance of chromosomes (SMC)-kleisin complexes organize chromosomal DNAs in all domains of life, with key roles in chromosome segregation, DNA repair and regulation of gene expression. They function through the entrapment and active translocation of DNA, but the underlying conformational changes are largely unclear. Using structural biology, mass spectrometry and cross-linking, we investigated the architecture of two evolutionarily distant SMC-kleisin complexes: MukBEF from Escherichia coli, and cohesin from Saccharomyces cerevisiae. We show that both contain a dynamic coiled-coil discontinuity, the elbow, near the middle of their arms that permits a folded conformation. Bending at the elbow brings into proximity the hinge dimerization domain and the head-kleisin module, situated at opposite ends of the arms. Our findings favour SMC activity models that include a large conformational change in the arms, such as a relative movement between DNA contact sites during DNA loading and translocation.

PubMed: 30833788
DOI: 10.1038/s41594-019-0196-z

実験手法

X-RAY DIFFRACTION (2.6 Å)