6H24

X-Ray Crystal Structure of the MSBI1.176 WH1 Domain, a Replication Protein Isolated from a Multiple Sclerosis Patient

Summary for 6H24

• Entry DOI: 10.2210/pdb6h24/pdb
• Descriptor: Replication protein (2 entities in total)
• Functional Keywords: repa, msbi1.176, wh1, sclerosis, replication
• Biological source: Sphinx1.76-related DNA
• Total number of polymer chains: 2
• Total formula weight: 30908.88

Authors

Kilic, T.,Popov, A.N.,Hansman, G.S. (deposition date: 2018-07-13, release date: 2019-05-15, Last modification date: 2024-05-15)

Primary citation

Kilic, T.,Popov, A.N.,Burk-Korner, A.,Koromyslova, A.,Zur Hausen, H.,Bund, T.,Hansman, G.S.
Structural analysis of a replication protein encoded by a plasmid isolated from a multiple sclerosis patient.
Acta Crystallogr D Struct Biol, 75:498-504, 2019

PubMed Abstract: Bovine meat and milk factors (BMMFs) are circular, single-stranded episomal DNAs that have been detected in bovine meat and milk products. BMMFs are thought to have roles in human malignant and degenerative diseases. BMMFs encode a replication initiator protein (Rep) that is actively transcribed and translated in human cells. In this study, a Rep WH1 domain encoded on a BMMF (MSBI1.176) isolated from a multiple sclerosis human brain sample was determined to 1.53 Å resolution using X-ray crystallography. The overall structure of the MSBI1.176 WH1 domain was remarkably similar to other Rep structures, despite having a low (28%) amino-acid sequence identity. The MSBI1.176 WH1 domain contained elements common to other Reps, including five α-helices, five β-strands and a hydrophobic pocket. These new findings suggest that the MSBI1.176 Rep might have comparable roles and functions to other known Reps of different origins.

PubMed: 31063152
DOI: 10.1107/S2059798319003991

Experimental method

X-RAY DIFFRACTION (1.53 Å)