6H0I

Solution structure of Melampsora larici-populina MlpP4.1

6H0I の概要

• エントリーDOI: 10.2210/pdb6h0i/pdb
• NMR情報: BMRB: 34298
• 分子名称: Secreted protein (1 entity in total)
• 機能のキーワード: cysteine knot protein, poplar rust, effector, unknown function
• 由来する生物種: Melampsora larici-populina 98AG31
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7978.62

構造登録者

Tsan, P.,Petre, B.,Hecker, A.,Rouhier, N.,Duplessis, S. (登録日: 2018-07-09, 公開日: 2018-08-22, 最終更新日: 2023-06-14)

主引用文献

de Guillen, K.,Lorrain, C.,Tsan, P.,Barthe, P.,Petre, B.,Saveleva, N.,Rouhier, N.,Duplessis, S.,Padilla, A.,Hecker, A.
Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds.
Sci Rep, 9:18084-18084, 2019

PubMed Abstract: Rust fungi are plant pathogens that secrete an arsenal of effector proteins interfering with plant functions and promoting parasitic infection. Effectors are often species-specific, evolve rapidly, and display low sequence similarities with known proteins. How rust fungal effectors function in host cells remains elusive, and biochemical and structural approaches have been scarcely used to tackle this question. In this study, we produced recombinant proteins of eleven candidate effectors of the leaf rust fungus Melampsora larici-populina in Escherichia coli. We successfully purified and solved the three-dimensional structure of two proteins, MLP124266 and MLP124017, using NMR spectroscopy. Although both MLP124266 and MLP124017 show no sequence similarity with known proteins, they exhibit structural similarities to knottins, which are disulfide-rich small proteins characterized by intricate disulfide bridges, and to nuclear transport factor 2-like proteins, which are molecular containers involved in a wide range of functions, respectively. Interestingly, such structural folds have not been reported so far in pathogen effectors, indicating that MLP124266 and MLP124017 may bear novel functions related to pathogenicity. Our findings show that sequence-unrelated effectors can adopt folds similar to known proteins, and encourage the use of biochemical and structural approaches to functionally characterize effector candidates.

PubMed: 31792250
DOI: 10.1038/s41598-019-53816-9

実験手法

SOLUTION NMR