6GZ6

Structure of a left-handed G-quadruplex

Summary for 6GZ6

Entry DOI	10.2210/pdb6gz6/pdb
Descriptor	DNA (27-MER), POTASSIUM ION (3 entities in total)
Functional Keywords	g-quadruplex, dna
Biological source	Homo sapiens
Total number of polymer chains	1
Total formula weight	8685.75
Authors	Bakalar, B.,Heddi, B.,Schmitt, E.,Mechulam, Y.,Phan, A.T. (deposition date: 2018-07-03, release date: 2019-04-24, Last modification date: 2024-01-17)
Primary citation	Bakalar, B.,Heddi, B.,Schmitt, E.,Mechulam, Y.,Phan, A.T. A Minimal Sequence for Left-Handed G-Quadruplex Formation. Angew.Chem.Int.Ed.Engl., 58:2331-2335, 2019 Cited by PubMed Abstract: Recently, we observed the first example of a left-handed G-quadruplex structure formed by natural DNA, named Z-G4. We analysed the Z-G4 structure and inspected its primary 28-nt sequence in order to identify motifs that convey the unique left-handed twist. Using circular dichroism spectroscopy, NMR spectroscopy, and X-ray crystallography, we revealed a minimal sequence motif of 12 nt (GTGGTGGTGGTG) for formation of the left-handed DNA G-quadruplex, which is found to be highly abundant in the human genome. A systematic analysis of thymine loop mutations revealed a moderate sequence tolerance, which would further broaden the space of sequences prone to left-handed G-quadruplex formation. PubMed: 30481397 DOI: 10.1002/anie.201812628 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.006 Å)

Structure validation