6GYM

Structure of a yeast closed complex with distorted DNA (CCdist)

6GYM の概要

• エントリーDOI: 10.2210/pdb6gym/pdb
• EMDBエントリー: 0092
• 分子名称: General transcription and DNA repair factor IIH helicase subunit XPD,General transcription and DNA repair factor IIH helicase subunit XPD,DNA repair helicase RAD3,DNA repair helicase RAD3,General transcription and DNA repair factor IIH helicase subunit XPD, DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II subunit RPB3, ... (34 entities in total)
• 機能のキーワード: rna polymerase ii, transcription initiation, promoter opening, transcription
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 31
• 化学式量合計: 1297092.70

構造登録者

Dienemann, C.,Schwalb, B.,Schilbach, S.,Cramer, P. (登録日: 2018-06-30, 公開日: 2018-12-05, 最終更新日: 2024-11-13)

主引用文献

Dienemann, C.,Schwalb, B.,Schilbach, S.,Cramer, P.
Promoter Distortion and Opening in the RNA Polymerase II Cleft.
Mol. Cell, 73:97-106.e4, 2019

PubMed Abstract: Transcription initiation requires opening of promoter DNA in the RNA polymerase II (Pol II) pre-initiation complex (PIC), but it remains unclear how this is achieved. Here we report the cryo-electron microscopic (cryo-EM) structure of a yeast PIC that contains underwound, distorted promoter DNA in the closed Pol II cleft. The DNA duplex axis is offset at the upstream edge of the initially melted DNA region (IMR) where DNA opening begins. Unstable IMRs are found in a subset of yeast promoters that we show can still initiate transcription after depletion of the transcription factor (TF) IIH (TFIIH) translocase Ssl2 (XPB in human) from the nucleus in vivo. PIC-induced DNA distortions may thus prime the IMR for melting and may explain how unstable IMRs that are predicted in promoters of Pol I and Pol III can open spontaneously. These results suggest that DNA distortion in the polymerase cleft is a general mechanism that contributes to promoter opening.

PubMed: 30472190
DOI: 10.1016/j.molcel.2018.10.014

実験手法

ELECTRON MICROSCOPY (6.7 Å)