6GXZ

Crystal structure of the human RPAP3(TPR2)-PIH1D1(CS) complex

Summary for 6GXZ

• Entry DOI: 10.2210/pdb6gxz/pdb
• Descriptor: RNA polymerase II-associated protein 3, PIH1 domain-containing protein 1, CALCIUM ION, ... (4 entities in total)
• Functional Keywords: cochaperone, complex, tpr domain, chaperone
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 58184.75

Authors

Henri, J.,Quinternet, M.,Manival, X.,Chagot, M.-E.,Charpentier, B.,Meyer, P. (deposition date: 2018-06-27, release date: 2018-08-08, Last modification date: 2024-01-17)

Primary citation

Henri, J.,Chagot, M.E.,Bourguet, M.,Abel, Y.,Terral, G.,Maurizy, C.,Aigueperse, C.,Georgescauld, F.,Vandermoere, F.,Saint-Fort, R.,Behm-Ansmant, I.,Charpentier, B.,Pradet-Balade, B.,Verheggen, C.,Bertrand, E.,Meyer, P.,Cianferani, S.,Manival, X.,Quinternet, M.
Deep Structural Analysis of RPAP3 and PIH1D1, Two Components of the HSP90 Co-chaperone R2TP Complex.
Structure, 26:1196-1209.e8, 2018

PubMed Abstract: RPAP3 and PIH1D1 are part of the HSP90 co-chaperone R2TP complex involved in the assembly process of many molecular machines. In this study, we performed a deep structural investigation of the HSP binding abilities of the two TPR domains of RPAP3. We combined 3D NMR, non-denaturing MS, and ITC techniques with Y2H, IP-LUMIER, FRET, and ATPase activity assays and explain the fundamental role played by the second TPR domain of RPAP3 in the specific recruitment of HSP90. We also established the 3D structure of an RPAP3:PIH1D1 sub-complex demonstrating the need for a 34-residue insertion, specific of RPAP3 isoform 1, for the tight binding of PIH1D1. We also confirm the existence of a complex lacking PIH1D1 in human cells (R2T), which shows differential binding to certain clients. These results highlight similarities and differences between the yeast and human R2TP complexes, and document the diversification of this family of co-chaperone complexes in human.

PubMed: 30033218
DOI: 10.1016/j.str.2018.06.002

Experimental method

X-RAY DIFFRACTION (2.965 Å)