6GXS

Crystal structure of CV39L lectin from Chromobacterium violaceum at 1.8 A resolution

6GXS の概要

• エントリーDOI: 10.2210/pdb6gxs/pdb
• 分子名称: CV39L lectin, SULFATE ION, 1,2-ETHANEDIOL, ... (6 entities in total)
• 機能のキーワード: lectin, sugar binding protein
• 由来する生物種: Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 160982.28

構造登録者

Sykorova, P.,Novotna, J.,Demo, G.,Pompidor, G.,Dubska, E.,Komarek, J.,Fujdiarova, E.,Haronikova, L.,Varrot, A.,Imberty, A.,Shilova, N.,Bovin, N.,Pokorna, M.,Wimmerova, M. (登録日: 2018-06-27, 公開日: 2019-12-04, 最終更新日: 2024-05-15)

主引用文献

Sykorova, P.,Novotna, J.,Demo, G.,Pompidor, G.,Dubska, E.,Komarek, J.,Fujdiarova, E.,Houser, J.,Haronikova, L.,Varrot, A.,Shilova, N.,Imberty, A.,Bovin, N.,Pokorna, M.,Wimmerova, M.
Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed beta-propeller fold.
Int.J.Biol.Macromol., 152:1113-1124, 2020

PubMed Abstract: Burkholderia pseudomallei and Chromobacterium violaceum are bacteria of tropical and subtropical soil and water that occasionally cause fatal infections in humans and animals. Microbial lectins mediate the adhesion of organisms to host cells, which is the first phase in the development of infection. Here we report the discovery of two novel lectins from the above-mentioned bacteria - BP39L and CV39L. The crystal structures revealed that the lectins possess a seven-bladed β-propeller fold. Functional studies conducted on a series of oligo- and polysaccharides confirmed the preference of BP39L for mannosylated saccharides and CV39L for rather more complex polysaccharides with a monosaccharide preference for β-l-fucose. The presented data indicate that the proteins belong to a currently unknown family of lectins.

PubMed: 31751748
DOI: 10.1016/j.ijbiomac.2019.10.200

実験手法

X-RAY DIFFRACTION (1.8 Å)