6GXP

Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP(RF3-only)

これはPDB形式変換不可エントリーです。

6GXP の概要

• エントリーDOI: 10.2210/pdb6gxp/pdb
• 関連するPDBエントリー: 6GWT 6GXM 6GXN 6GXO
• EMDBエントリー: 0083
• 分子名称: 23S ribosomal RNA, 50S ribosomal protein L13, 50S ribosomal protein L14, ... (56 entities in total)
• 機能のキーワード: single particle cryo-em, gtpase, ribosome, release factor rf1, rf2, rf3, subunit rotation, translation termination
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 55
• 化学式量合計: 2194634.44

構造登録者

Graf, M.,Huter, P.,Maracci, C.,Peterek, M.,Rodnina, M.V.,Wilson, D.N. (登録日: 2018-06-27, 公開日: 2018-08-15)

主引用文献

Graf, M.,Huter, P.,Maracci, C.,Peterek, M.,Rodnina, M.V.,Wilson, D.N.
Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1.
Nat Commun, 9:3053-3053, 2018

PubMed Abstract: During translation termination in bacteria, the release factors RF1 and RF2 are recycled from the ribosome by RF3. While high-resolution structures of the individual termination factors on the ribosome exist, direct structural insight into how RF3 mediates dissociation of the decoding RFs has been lacking. Here we have used the Apidaecin 137 peptide to trap RF1 together with RF3 on the ribosome and visualize an ensemble of termination intermediates using cryo-electron microscopy. Binding of RF3 to the ribosome induces small subunit (SSU) rotation and swivelling of the head, yielding intermediate states with shifted P-site tRNAs and RF1 conformations. RF3 does not directly eject RF1 from the ribosome, but rather induces full rotation of the SSU that indirectly dislodges RF1 from its binding site. SSU rotation is coupled to the accommodation of the GTPase domain of RF3 on the large subunit (LSU), thereby promoting GTP hydrolysis and dissociation of RF3 from the ribosome.

PubMed: 30076302
DOI: 10.1038/s41467-018-05465-1

実験手法

ELECTRON MICROSCOPY (4.4 Å)