6GXH

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography: FAcD 0MS after reaction initiation

Summary for 6GXH

• Entry DOI: 10.2210/pdb6gxh/pdb
• Descriptor: Fluoroacetate dehalogenase (2 entities in total)
• Functional Keywords: time-resolved crystallography, serial synchrotron crystallography (ssx), pump-probe experiment, structural enzymology, hydrolase
• Biological source: Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
• Total number of polymer chains: 2
• Total formula weight: 68147.32

Authors

Schulz, E.C.,Mehrabi, P.,Mueller-Werkmeister, H.,Tellkamp, F.,Stuart, W.,Persch, E.,De Gasparo, R.,Diederich, F.,Pai, E.F.,Miller, R.J.D. (deposition date: 2018-06-27, release date: 2018-10-17, Last modification date: 2024-01-17)

Primary citation

Schulz, E.C.,Mehrabi, P.,Muller-Werkmeister, H.M.,Tellkamp, F.,Jha, A.,Stuart, W.,Persch, E.,De Gasparo, R.,Diederich, F.,Pai, E.F.,Miller, R.J.D.
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.
Nat. Methods, 15:901-904, 2018

PubMed Abstract: We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

PubMed: 30377366
DOI: 10.1038/s41592-018-0180-2

Experimental method

X-RAY DIFFRACTION (1.802 Å)