6GXD

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography: FAcD752MS after reaction initiation

6GXD の概要

• エントリーDOI: 10.2210/pdb6gxd/pdb
• 関連するPDBエントリー: 5O2I 6FSX
• 分子名称: fluoroacetate dehalogenase, fluoroacetic acid, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: time-resolved crystallography, serial synchrotron crystallography (ssx), pump-probe experiment, structural enzymology, dna binding protein
• 由来する生物種: Rhodopseudomonas palustris
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68296.27

構造登録者

Schulz, E.C.,Mehrabi, P.,Mueller-Werkmeister, H.,Tellkamp, F.,Stuart, W.,Persch, E.,De Gasparo, R.,Diederich, F.,Pai, E.F.,Miller, R.J.D. (登録日: 2018-06-27, 公開日: 2018-10-17, 最終更新日: 2024-01-17)

主引用文献

Schulz, E.C.,Mehrabi, P.,Muller-Werkmeister, H.M.,Tellkamp, F.,Jha, A.,Stuart, W.,Persch, E.,De Gasparo, R.,Diederich, F.,Pai, E.F.,Miller, R.J.D.
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.
Nat. Methods, 15:901-904, 2018

PubMed Abstract: We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals.

PubMed: 30377366
DOI: 10.1038/s41592-018-0180-2

実験手法

X-RAY DIFFRACTION (1.8 Å)