6GX5

Narrow Pick Filament from Pick's disease brain

6GX5 の概要

• エントリーDOI: 10.2210/pdb6gx5/pdb
• EMDBエントリー: 0077
• 分子名称: Microtubule-associated protein tau (1 entity in total)
• 機能のキーワード: tau protein, tau, pick's disease, tauopathy, neurodegenerative disease, proteinopathy, amyloid, filament, helical, pick body, fibril, neurodegeneration, mapt, microtubule-associated protein tau, protein fibril
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 30407.00

構造登録者

Falcon, B.,Zhang, W.,Murzin, A.G.,Murshudov, G.,Garringer, H.J.,Vidal, R.,Crowther, R.A.,Ghetti, B.,Scheres, S.H.W.,Goedert, M. (登録日: 2018-06-26, 公開日: 2018-09-12, 最終更新日: 2024-07-10)

主引用文献

Falcon, B.,Zhang, W.,Murzin, A.G.,Murshudov, G.,Garringer, H.J.,Vidal, R.,Crowther, R.A.,Ghetti, B.,Scheres, S.H.W.,Goedert, M.
Structures of filaments from Pick's disease reveal a novel tau protein fold.
Nature, 561:137-140, 2018

PubMed Abstract: The ordered assembly of tau protein into abnormal filamentous inclusions underlies many human neurodegenerative diseases. Tau assemblies seem to spread through specific neural networks in each disease, with short filaments having the greatest seeding activity. The abundance of tau inclusions strongly correlates with disease symptoms. Six tau isoforms are expressed in the normal adult human brain-three isoforms with four microtubule-binding repeats each (4R tau) and three isoforms that lack the second repeat (3R tau). In various diseases, tau filaments can be composed of either 3R or 4R tau, or of both. Tau filaments have distinct cellular and neuroanatomical distributions, with morphological and biochemical differences suggesting that they may be able to adopt disease-specific molecular conformations. Such conformers may give rise to different neuropathological phenotypes, reminiscent of prion strains. However, the underlying structures are not known. Using electron cryo-microscopy, we recently reported the structures of tau filaments from patients with Alzheimer's disease, which contain both 3R and 4R tau. Here we determine the structures of tau filaments from patients with Pick's disease, a neurodegenerative disorder characterized by frontotemporal dementia. The filaments consist of residues Lys254-Phe378 of 3R tau, which are folded differently from the tau filaments in Alzheimer's disease, establishing the existence of conformers of assembled tau. The observed tau fold in the filaments of patients with Pick's disease explains the selective incorporation of 3R tau in Pick bodies, and the differences in phosphorylation relative to the tau filaments of Alzheimer's disease. Our findings show how tau can adopt distinct folds in the human brain in different diseases, an essential step for understanding the formation and propagation of molecular conformers.

PubMed: 30158706
DOI: 10.1038/s41586-018-0454-y

実験手法

ELECTRON MICROSCOPY (3.2 Å)