6GX4

The molybdenum storage protein: with ATP/Mn2+ and with POM clusters formed under in vitro conditions

6GX4 の概要

• エントリーDOI: 10.2210/pdb6gx4/pdb
• 分子名称: Molybdenum storage protein subunit beta, Molybdenum storage protein subunit alpha, ADENOSINE-5'-TRIPHOSPHATE, ... (9 entities in total)
• 機能のキーワード: molybdate storage, polyoxometalate clusters, atp consumption, metal binding protein
• 由来する生物種: Azotobacter vinelandii (strain DJ / ATCC BAA-1303); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61959.53

構造登録者

Poppe, J.,Bruenle, S.,Hail, R.,Ermler, E. (登録日: 2018-06-26, 公開日: 2018-12-05, 最終更新日: 2024-01-17)

主引用文献

Poppe, J.,Brunle, S.,Hail, R.,Wiesemann, K.,Schneider, K.,Ermler, U.
The Molybdenum Storage Protein: A soluble ATP hydrolysis-dependent molybdate pump.
FEBS J., 285:4602-4616, 2018

PubMed Abstract: A continuous FeMo cofactor supply for nitrogenase maturation is ensured in Azotobacter vinelandii by developing a cage-like molybdenum storage protein (MoSto) capable to store ca. 120 molybdate molecules ( ) as discrete polyoxometalate (POM) clusters. To gain mechanistic insight into this process, MoSto was characterized by Mo and ATP/ADP content, structural, and kinetic analysis. We defined three functionally relevant states specified by the presence of both ATP/ADP and POM clusters (MoSto ), of only ATP/ADP (MoSto ) and of neither ATP/ADP nor POM clusters (MoSto ), respectively. POM clusters are only produced when ATP is hydrolyzed to ADP and phosphate. V was ca. 13 μmol ·min ·mg and K for molybdate and ATP/Mg in the low micromolar range. ATP hydrolysis presumably proceeds at subunit α, inferred from a highly occupied α-ATP/Mg and a weaker occupied β-ATP/no Mg -binding site found in the MoSto structure. Several findings indicate that POM cluster storage is separated into a rapid ATP hydrolysis-dependent molybdate transport across the protein cage wall and a slow molybdate assembly induced by combined auto-catalytic and protein-driven processes. The cage interior, the location of the POM cluster depot, is locked in all three states and thus not rapidly accessible for molybdate from the outside. Based on V , the entire Mo storage process should be completed in less than 10 s but requires, according to the molybdate content analysis, ca. 15 min. Long-time incubation of MoSto with nonphysiological high molybdate amounts implicates an equilibrium in and outside the cage and POM cluster self-formation without ATP hydrolysis. DATABASES: The crystal structures MoSto in the MoSto-F6, MoSto-F7, MoSto , MoSto , and MoSto-F1 states were deposited to PDB under the accession numbers PDB 6GU5, 6GUJ, 6GWB, 6GWV, and 6GX4.

PubMed: 30367742
DOI: 10.1111/febs.14684

実験手法

X-RAY DIFFRACTION (1.9 Å)