Transient state structure of CRISPR-Cpf1 (Cas12a) I3 conformation

Summary for 6GTE

EMDB information0063
DescriptorCRISPR-associated endonuclease Cas12a, RNA (29-MER), DNA (5'-D(P*AP*TP*GP*AP*CP*TP*TP*CP*TP*CP*TP*AP*AP*CP*AP*AP*GP*CP*TP*CP*G)-3'), ... (4 entities in total)
Functional Keywordscrispr genome editing ribonucleoprotein complex, hydrolase
Biological sourceFrancisella tularensis subsp. novicida (strain U112)
Total number of polymer chains4
Total molecular weight203280.74
Montoya, G.,Mesa, P.,Stella, S. (deposition date: 2018-06-18, release date: 2018-12-19, Last modification date: 2018-12-26)
Primary citation
Stella, S.,Mesa, P.,Thomsen, J.,Paul, B.,Alcon, P.,Jensen, S.B.,Saligram, B.,Moses, M.E.,Hatzakis, N.S.,Montoya, G.
Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Cell, 175:1856-1871.e21, 2018
PubMed: 30503205 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2018.10.045
MImport into Mendeley
Experimental method

Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone8 0.1% 1.5% 0.37MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures