6GSR

Single Particle Cryo-EM map of human Transferrin receptor 1 - H-Ferritin complex at 5.5 Angstrom resolution.

これはPDB形式変換不可エントリーです。

6GSR の概要

• エントリーDOI: 10.2210/pdb6gsr/pdb
• EMDBエントリー: 0046
• 分子名称: Ferritin heavy chain, Transferrin receptor protein 1 (2 entities in total)
• 機能のキーワード: transferrin receptor 1, ferritin, complex, single particle cryo-em, metal binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 650601.53

構造登録者

Testi, C.,Montemiglio, L.C.,Vallone, B.,Des Georges, A.,Boffi, A.,Mancia, F.,Baiocco, P. (登録日: 2018-06-15, 公開日: 2019-03-27, 最終更新日: 2024-11-13)

主引用文献

Montemiglio, L.C.,Testi, C.,Ceci, P.,Falvo, E.,Pitea, M.,Savino, C.,Arcovito, A.,Peruzzi, G.,Baiocco, P.,Mancia, F.,Boffi, A.,des Georges, A.,Vallone, B.
Cryo-EM structure of the human ferritin-transferrin receptor 1 complex.
Nat Commun, 10:1121-1121, 2019

PubMed Abstract: Human transferrin receptor 1 (CD71) guarantees iron supply by endocytosis upon binding of iron-loaded transferrin and ferritin. Arenaviruses and the malaria parasite exploit CD71 for cell invasion and epitopes on CD71 for interaction with transferrin and pathogenic hosts were identified. Here, we provide the molecular basis of the CD71 ectodomain-human ferritin interaction by determining the 3.9 Å resolution single-particle cryo-electron microscopy structure of their complex and by validating our structural findings in a cellular context. The contact surfaces between the heavy-chain ferritin and CD71 largely overlap with arenaviruses and Plasmodium vivax binding regions in the apical part of the receptor ectodomain. Our data account for transferrin-independent binding of ferritin to CD71 and suggest that select pathogens may have adapted to enter cells by mimicking the ferritin access gate.

PubMed: 30850661
DOI: 10.1038/s41467-019-09098-w

実験手法

ELECTRON MICROSCOPY (5.5 Å)