6GSJ

Structure of T. thermophilus 70S ribosome complex with mRNA, tRNAfMet and cognate tRNAThr in the A-site

これはPDB形式変換不可エントリーです。

6GSJ の概要

• エントリーDOI: 10.2210/pdb6gsj/pdb
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (59 entities in total)
• 機能のキーワード: translation, ribosome, decoding
• 由来する生物種: Thermus thermophilus HB8; 詳細
• タンパク質・核酸の鎖数: 107
• 化学式量合計: 4511212.92

構造登録者

Rozov, A.,Yusupov, M.,Yusupova, G. (登録日: 2018-06-14, 公開日: 2018-07-04, 最終更新日: 2024-01-17)

主引用文献

Rozov, A.,Wolff, P.,Grosjean, H.,Yusupov, M.,Yusupova, G.,Westhof, E.
Tautomeric G•U pairs within the molecular ribosomal grip and fidelity of decoding in bacteria.
Nucleic Acids Res., 46:7425-7435, 2018

PubMed Abstract: We report new crystallographic structures of Thermus thermophilus ribosomes complexed with long mRNAs and native Escherichia coli tRNAs. They complete the full set of combinations of Watson-Crick G•C and miscoding G•U pairs at the first two positions of the codon-anticodon duplex in ribosome functional complexes. Within the tight decoding center, miscoding G•U pairs occur, in all combinations, with a non-wobble geometry structurally indistinguishable from classical coding Watson-Crick pairs at the same first two positions. The contacts with the ribosomal grip surrounding the decoding center are all quasi-identical, except in the crowded environment of the amino group of a guanosine at the second position; in which case a G in the codons may be preferred. In vivo experimental data show that the translational errors due to miscoding by G•U pairs at the first two positions are the most frequently encountered ones, especially at the second position and with a G on the codon. Such preferred miscodings involve a switch from an A-U to a G•U pair in the tRNA/mRNA complex and very rarely from a G = C to a G•U pair. It is concluded that the frequencies of such occurrences are only weakly affected by the codon/anticodon structures but depend mainly on the stability and lifetime of the complex, the modifications present in the anticodon loop, especially those at positions 34 and 37, in addition to the relative concentration of cognate/near-cognate tRNA species present in the cellular tRNA pool.

PubMed: 29931292
DOI: 10.1093/nar/gky547

実験手法

X-RAY DIFFRACTION (2.96 Å)