6GQA

Cell division regulator S. pneumoniae GpsB

6GQA の概要

• エントリーDOI: 10.2210/pdb6gqa/pdb
• 分子名称: Cell cycle protein GpsB (2 entities in total)
• 機能のキーワード: bacterial cell division regulator, peptidoglycan synthesis regulator, penicillin binding protein interaction partner, cell cycle
• 由来する生物種: Streptococcus pneumoniae R6
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 29012.54

構造登録者

Lewis, R.J.,Rutter, Z.J. (登録日: 2018-06-07, 公開日: 2019-01-23, 最終更新日: 2024-01-17)

主引用文献

Cleverley, R.M.,Rutter, Z.J.,Rismondo, J.,Corona, F.,Tsui, H.T.,Alatawi, F.A.,Daniel, R.A.,Halbedel, S.,Massidda, O.,Winkler, M.E.,Lewis, R.J.
The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes.
Nat Commun, 10:261-261, 2019

PubMed Abstract: Bacterial growth and cell division requires precise spatiotemporal regulation of the synthesis and remodelling of the peptidoglycan layer that surrounds the cytoplasmic membrane. GpsB is a cytosolic protein that affects cell wall synthesis by binding cytoplasmic mini-domains of peptidoglycan synthases to ensure their correct subcellular localisation. Here, we describe critical structural features for the interaction of GpsB with peptidoglycan synthases from three bacterial species (Bacillus subtilis, Listeria monocytogenes and Streptococcus pneumoniae) and suggest their importance for cell wall growth and viability in L. monocytogenes and S. pneumoniae. We use these structural motifs to identify novel partners of GpsB in B. subtilis and extend the members of the GpsB interactome in all three bacterial species. Our results support that GpsB functions as an adaptor protein that mediates the interaction between membrane proteins, scaffolding proteins, signalling proteins and enzymes to generate larger protein complexes at specific sites in a bacterial cell cycle-dependent manner.

PubMed: 30651563
DOI: 10.1038/s41467-018-08056-2

実験手法

X-RAY DIFFRACTION (1.9 Å)